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Akt1 as a putative regulator of Hox genes

Authors
 Kyoung-Ah Kong  ;  Heejei Yoon  ;  Myoung Hee Kim 
Citation
 Gene, Vol.513(2) : 287-291, 2013 
Journal Title
 Gene 
ISSN
 0378-1119 
Issue Date
2013
MeSH
Animals ; Cells, Cultured ; Computer Simulation ; CpG Islands ; DNA Methylation ; Fibroblasts ; Gene Expression Regulation ; Genes, Homeobox* ; Genes, Regulator* ; Mice ; Proto-Oncogene Proteins c-akt/genetics* ; Proto-Oncogene Proteins c-akt/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Up-Regulation
Keywords
Hox gene ; Akt1 ; In silico analysis ; DNA methylation
Abstract
In mammals, precise spatiotemporal expressions of Hox genes control the main body axis during embryogenesis. However, the mechanism by which Hox genes are regulated is poorly understood. To discover the putative regulator of Hox genes, in silico analyses were performed using GEO profiles, and Akt1 emerged as a candidate regulator of Hox genes in E13.5 MEFs. The results of the RT-PCR showed that 5′ Hoxc genes, including ncRNA were upregulated in Akt1 null MEF. Combined bisulfite restriction analysis (COBRA) and bisulfite sequencing showed that the CpG island of a 5′ Hoxc gene was hypomethylated in Akt1 null cells. These results indicate that Hox expression could be controlled by the function of Akt1 through epigenetic modification such as DNA methylation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0378111912013121
DOI
10.1016/j.gene.2012.10.034
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kong, Kyoung Ah(공경아)
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86135
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