Peri-implantitis is a major threat to implant longevity, requiring timely diagnosis and effective treatment. This case report describes two clinical cases where quantitative light-induced fluorescence (QLF) was used to detect and evaluate peri-implant biofilm. The first involved a medically compromised patient treated non-surgically using ultrasonic scalers, NiTi brushes, and an air-powder abrasive device. The second case required surgical intervention with flap elevation and bone grafting. In both cases, QLF enabled real-time visualization of biofilm accumulation and removal. Fluorescence intensity markedly decreased after treatment, indicating improved implant surface cleanliness. QLF allowed detection of residual biofilm that was not easily identified through traditional visual inspection, supporting more thorough decontamination. These findings suggest that QLF is a useful adjunctive tool for the diagnosis and evaluation of peri-implantitis. By enabling precise visualization of biofilm, QLF enhances conventional assessments and may contribute to better treatment outcomes and long-term implant maintenance.