Peri-implantitis is a major threat to implant longevity, requiring timely diagnosis and effective treatment. This case report describes two clinical cases where quantitative light-induced fluorescence (QLF) was used to detect and evaluate peri-implant biofilm. The first involved a medically compromised patient treated non-surgically using ultrasonic scalers, NiTi brushes, and an air-powder abrasive device. The second case required surgical intervention with flap elevation and bone grafting. In both cases, QLF enabled real-time visualization of biofilm accumulation and removal. Fluorescence intensity markedly decreased after treatment, indicating improved im- plant surface cleanliness. QLF allowed detection of residual biofilm that was not easily identified through tradi- tional visual inspection, supporting more thorough decontamination. These findings suggest that QLF is a useful adjunctive tool for the diagnosis and evaluation of peri-implantitis. By enabling precise visualization of biofilm, QLF enhances conventional assessments and may contribute to better treatment outcomes and long-term implant maintenance. (J Korean Dent Assoc 2025; 63(6): 181-189)