Repositioning of ezetimibe for the treatment of idiopathic pulmonary fibrosis

Abstract

Background We previously identified ezetimibe, an inhibitor of Niemann - Pick C1 -like intracellular cholesterol transporter 1 and European Medicines Agency -approved lipid -lowering agent, as a potent autophagy activator. However, its efficacy against pulmonary fibrosis has not yet been evaluated. This study aimed to determine whether ezetimibe has therapeutic potential against idiopathic pulmonary fibrosis. Methods Primary lung fibroblasts isolated from both humans and mice were employed for mechanistic vitro experiments. mRNA sequencing of human lung fibroblasts and gene set enrichment analysis were performed to explore the therapeutic mechanism of ezetimibe. A bleomycin-induced pulmonary fibrosis mouse model was used to examine in vivo efficacy of the drug. Tandem fluorescent -tagged microtubuleassociated protein 1 light chain 3 transgenic mice were used to measure autophagic flux. Finally, the medical records of patients with idiopathic pulmonary fibrosis from three different hospitals were reviewed retrospectively, and analyses on survival and lung function were conducted to determine the benefits of ezetimibe. Results Ezetimibe inhibited myofibroblast differentiation by restoring the mechanistic target of rapamycin complex 1 - autophagy axis with fine control of intracellular cholesterol distribution. Serum response factor, potential autophagic substrate, was identified as a primary downstream effector in this process. Similarly, ezetimibe ameliorated bleomycin-induced pulmonary fibrosis in mice by inhibiting mechanistic target of rapamycin complex 1 activity and increasing autophagic flux, as observed in mouse lung samples. Patients with idiopathic pulmonary fibrosis who regularly used ezetimibe showed decreased rates of all -cause mortality and lung function decline. Conclusion Our study presents ezetimibe as a potential novel therapeutic for idiopathic pulmonary fibrosis.