발작야간혈색소뇨증에서 GPI 결핍세포 검출을 위한 유세포검사법: 국내 실태조사 및 검사법 지침 제언
Other Titles
Detection of GPI-deficient Cells in Paroxysmal Nocturnal Hemoglobinuria Patients Using Flow Cytometry: Current Status in Korea and Proposal for Testing Guidelines
Paroxysmal nocturnal hemoglobinuria ; Flow cytometry ; Practice guideline
Abstract
We performed an online survey to investigate the current status of detecting glycosylphosphatidylinositol (GPI)-deficient cells in patients with paroxysmal nocturnal hemoglobinuria (PNH) using flow cytometry in Korea. Questionnaires were sent to 29 laboratories performing flow cytometric analyses. The questionnaire included testing procedures, data analysis, reporting, assay validation, and quality control. The results demonstrated these factors varied among laboratories in Korea; thus, we propose a protocol to improve the quality of flow cytometric PNH testing based on international guidelines.