발작야간혈색소뇨증에서 GPI 결핍세포 검출을 위한 유세포검사법: 국내 실태조사 및 검사법 지침 제언

Abstract

We performed an online survey to investigate the current status of detecting glycosylphosphatidylinositol (GPI)-deficient cells in patients with paroxysmal nocturnal hemoglobinuria (PNH) using flow cytometry in Korea. Questionnaires were sent to 29 laboratories performing flow cytometric analyses. The questionnaire included testing procedures, data analysis, reporting, assay validation, and quality control. The results demonstrated these factors varied among laboratories in Korea; thus, we propose a protocol to improve the quality of flow cytometric PNH testing based on international guidelines.