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Effect of high-dose vitamin C on renal ischemia-reperfusion injury

Authors
 Seo Hee Ko  ;  Ji Hae Jun  ;  Ju Eun Oh  ;  Eunah Shin  ;  Young-Lan Kwak  ;  Jae-Kwang Shim 
Citation
 BIOMEDICINE & PHARMACOTHERAPY, Vol.173 : 116407, 2024-03 
Journal Title
BIOMEDICINE & PHARMACOTHERAPY
ISSN
 0753-3322 
Issue Date
2024-03
MeSH
Acute Kidney Injury* / metabolism ; Animals ; Antineoplastic Agents* / pharmacology ; Ascorbic Acid / metabolism ; Ascorbic Acid / pharmacology ; Ascorbic Acid / therapeutic use ; Creatinine ; Humans ; Inflammation / metabolism ; Ischemia / metabolism ; Kidney ; Oxidative Stress ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury* / pathology
Keywords
Acute kidney injury ; Ascorbic acid ; Ischemia-reperfusion injury ; Vitamin C
Abstract
Acute kidney injury frequently occurs after cardiac surgery, and is primarily attributed to renal ischemia?reperfusion (I/R) injury and inflammation from surgery and cardiopulmonary bypass. Vitamin C, an antioxi?dant that is often depleted in critically ill patients, could potentially mitigate I/R-induced oxidative stress at high doses. We investigated the effectiveness of high-dose vitamin C in preventing I/R-induced renal injury. The ideal time and optimal dosage for administration were determined in a two-phase experiment on Sprague–Dawley rats.

The rats were assigned to four groups: sham, IRC (I/R + saline), and pre- and post-vitC (vitamin C before and after I/R, respectively), with vitamin C administered at 200 mg/kg. Additional groups were examined for dose modification based on the optimal timing determined: V100, V200, and V300 (100, 200, and 300 mg/kg, respectively). Renal I/R was achieved through 45 min of ischemia followed by 24 h of reperfusion. Vitamin C

administration during reperfusion significantly reduced renal dysfunction and tubular damage, more than pre?ischemic administration. Doses of 100 and 200 mg/kg during reperfusion reduced oxidative stress markers, including myeloperoxidase and inflammatory responses by decreasing high mobility group box 1 release and nucleotide-binding and oligomerization domain-like receptor 3 inflammasome. Overall beneficial effect was most prominent with 200 mg/kg. The 300 mg/kg dose, however, showed no additional benefits over the IRC group regarding serum blood urea nitrogen and creatinine levels and histological evaluation. During reperfusion, high-dose vitamin C administration (200 mg/kg) significantly decreased renal I/R injury by effectively attenu?ating the major triggers of oxidative stress and inflammation.
Files in This Item:
T202402210.pdf Download
DOI
10.1016/j.biopha.2024.116407
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Ko, Seo Hee(고서희) ORCID logo https://orcid.org/0000-0001-8402-5624
Kwak, Young Lan(곽영란) ORCID logo https://orcid.org/0000-0002-2984-9927
Shin, Eunah(신은아)
Shim, Jae Kwang(심재광) ORCID logo https://orcid.org/0000-0001-9093-9692
Jun, Ji Hae(전지혜) ORCID logo https://orcid.org/0000-0002-8080-0715
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198884
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