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Egg microneedles for transdermal vaccination of inactivated influenza virus

Authors
 Geonwoo Kang  ;  Minkyung Kim  ;  Youjin Lee  ;  Huisuk Yang  ;  Baik-Lin Seong  ;  Hyungil Jung 
Citation
 BIOMATERIALS SCIENCE, Vol.12(4) : 907-918, 2024-02 
Journal Title
BIOMATERIALS SCIENCE
ISSN
 2047-4830 
Issue Date
2024-02
MeSH
Animals ; Drug Delivery Systems ; Influenza Vaccines* ; Injections, Intradermal ; Mice ; Orthomyxoviridae* ; Skin ; Vaccination / methods
Abstract
The use of dissolving microneedles (DMNs) is a drug delivery technique in which drug dissolution occurs once it is administered into the skin. The skin is a remarkable site for vaccination due to its significant immunologic properties. Compared to the traditional hypodermic intramuscular (IM) injection, vaccination via DMN does not require cold chains and allows for minimal invasive drug delivery. On account of the significance of skin vaccination, preceding studies have been conducted to elucidate the importance of the DMN technology in vaccination. Most of these studies focused on formulations that maintain the activity of the vaccine, so formulations designed to be specific to the mechanical properties of the microneedle could not be used together independently. In this study, we have developed influenza vaccine loaded egg microneedles (EMN) and characterized the specificity of layer-specific functions of EMN by distinguishing between formulations that can maintain the activity of the vaccine and have the mechanical strength. By the use of in vitro tests such as ELISA and SRID assays, we quantitively evaluated the antigen activity of the formulation candidates to be 87% and 91%, respectively. In vivo tests were also conducted as mouse groups were inoculated with the formulation constructed into egg microneedles (FLU-EMN) to determine the protective efficacy against infection. The results demonstrated that FLU-EMN with functionalized formulations successfully enabled protective immune response even with a fractional dose compared to IM injection.
Full Text
https://pubs.rsc.org/en/content/articlelanding/2024/bm/d3bm01635h
DOI
10.1039/d3bm01635h
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Seong, Baik L(성백린)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198732
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