Cited 3 times in

Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid

Authors
 Sojeong Lee  ;  Eunjung Kim  ;  Chae-Eun Moon  ;  Chaewon Park  ;  Jong-Woo Lim  ;  Minseok Baek  ;  Moo-Kwang Shin  ;  Jisun Ki  ;  Hanna Cho  ;  Yong Woo Ji  ;  Seungjoo Haam 
Citation
 NATURE COMMUNICATIONS, Vol.14(1) : 8153, 2023-12 
Journal Title
NATURE COMMUNICATIONS
Issue Date
2023-12
MeSH
Alzheimer Disease* / metabolism ; Amyloid beta-Peptides ; Biomarkers / metabolism ; Early Diagnosis ; Humans ; Immunoassay ; Proteomics ; Reproducibility of Results
Abstract
Accurate diagnosis of Alzheimer’s disease (AD) in its earliest stage can prevent the disease and delay the symptoms. Therefore, more sensitive, non-invasive, and simple screening tools are required for the early diagnosis and monitoring of AD. Here, we design a self-assembled nanoparticle-mediated amplified fluorogenic immunoassay (SNAFIA) consisting of magnetic and fluorophore-loaded polymeric nanoparticles. Using a discovery cohort of 21 subjects, proteomic analysis identifies adenylyl cyclase-associated protein 1 (CAP1) as a potential tear biomarker. The SNAFIA demonstrates a low detection limit (236 aM), good reliability (R2 = 0.991), and a wide analytical range (0.320–1000 fM) for CAP1 in tear fluid. Crucially, in the verification phase with 39 subjects, SNAFIA discriminates AD patients from healthy controls with 90% sensitivity and 100% specificity in under an hour. Utilizing tear fluid as a liquid biopsy, SNAFIA could potentially aid in long-term care planning, improve clinical trial efficiency, and accelerate therapeutic development for AD. © 2023, The Author(s).
Files in This Item:
T202400833.pdf Download
DOI
10.1038/s41467-023-43995-5
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Cho, Hanna(조한나) ORCID logo https://orcid.org/0000-0001-5936-1546
Ji, Yong Woo(지용우) ORCID logo https://orcid.org/0000-0002-7211-6278
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198046
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links