Cited 8 times in
Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid
DC Field | Value | Language |
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dc.contributor.author | 조한나 | - |
dc.contributor.author | 지용우 | - |
dc.date.accessioned | 2024-02-15T06:48:26Z | - |
dc.date.available | 2024-02-15T06:48:26Z | - |
dc.date.issued | 2023-12 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/198046 | - |
dc.description.abstract | Accurate diagnosis of Alzheimer’s disease (AD) in its earliest stage can prevent the disease and delay the symptoms. Therefore, more sensitive, non-invasive, and simple screening tools are required for the early diagnosis and monitoring of AD. Here, we design a self-assembled nanoparticle-mediated amplified fluorogenic immunoassay (SNAFIA) consisting of magnetic and fluorophore-loaded polymeric nanoparticles. Using a discovery cohort of 21 subjects, proteomic analysis identifies adenylyl cyclase-associated protein 1 (CAP1) as a potential tear biomarker. The SNAFIA demonstrates a low detection limit (236 aM), good reliability (R2 = 0.991), and a wide analytical range (0.320–1000 fM) for CAP1 in tear fluid. Crucially, in the verification phase with 39 subjects, SNAFIA discriminates AD patients from healthy controls with 90% sensitivity and 100% specificity in under an hour. Utilizing tear fluid as a liquid biopsy, SNAFIA could potentially aid in long-term care planning, improve clinical trial efficiency, and accelerate therapeutic development for AD. © 2023, The Author(s). | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Pub. Group | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Alzheimer Disease* / metabolism | - |
dc.subject.MESH | Amyloid beta-Peptides | - |
dc.subject.MESH | Biomarkers / metabolism | - |
dc.subject.MESH | Early Diagnosis | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoassay | - |
dc.subject.MESH | Proteomics | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.title | Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurology (신경과학교실) | - |
dc.contributor.googleauthor | Sojeong Lee | - |
dc.contributor.googleauthor | Eunjung Kim | - |
dc.contributor.googleauthor | Chae-Eun Moon | - |
dc.contributor.googleauthor | Chaewon Park | - |
dc.contributor.googleauthor | Jong-Woo Lim | - |
dc.contributor.googleauthor | Minseok Baek | - |
dc.contributor.googleauthor | Moo-Kwang Shin | - |
dc.contributor.googleauthor | Jisun Ki | - |
dc.contributor.googleauthor | Hanna Cho | - |
dc.contributor.googleauthor | Yong Woo Ji | - |
dc.contributor.googleauthor | Seungjoo Haam | - |
dc.identifier.doi | 10.1038/s41467-023-43995-5 | - |
dc.contributor.localId | A03920 | - |
dc.contributor.localId | A03967 | - |
dc.relation.journalcode | J02293 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.pmid | 38071202 | - |
dc.contributor.alternativeName | Cho, Hanna | - |
dc.contributor.affiliatedAuthor | 조한나 | - |
dc.contributor.affiliatedAuthor | 지용우 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 8153 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, Vol.14(1) : 8153, 2023-12 | - |
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