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Clinical utility of quantitative immunoassays and surrogate virus neutralization tests for predicting neutralizing activity against the SARS-CoV-2 Omicron BA.1 and BA.5 variants

Authors
 Beomki Lee  ;  Jae-Hoon Ko  ;  Yong Chan Kim  ;  Jin Yang Baek  ;  Yoon Soo Park  ;  Kyoung-Ho Song  ;  Eu Suk Kim  ;  Kyoung Hwa Lee  ;  Young Goo Song  ;  Jin Young Ahn  ;  Jun Yong Choi  ;  Won Suk Choi  ;  Seongman Bae  ;  Sung-Han Kim  ;  Hye Won Jeong  ;  Young Jae Lee  ;  Hye-Jin Kim  ;  Ju-Yeon Choi  ;  Byoungguk Kim  ;  Shin-Woo Kim  ;  Ki Tae Kwon  ;  Kyong Ran Peck  ;  Eun-Suk Kang 
Citation
 JOURNAL OF MEDICAL VIROLOGY, Vol.95(12) : e29329, 2023-12 
Journal Title
JOURNAL OF MEDICAL VIROLOGY
ISSN
 0146-6615 
Issue Date
2023-12
MeSH
Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19* / diagnosis ; Humans ; Immunoassay ; Neutralization Tests ; SARS-CoV-2* / genetics
Keywords
COVID-19 ; SARS-CoV-2 variants ; immunoassay ; neutralizing antibodies
Abstract
Developing new antibody assays for emerging SARS-CoV-2 variants is challenging. SARS-CoV-2 surrogate virus neutralization tests (sVNT) targeting Omicron BA.1 and BA.5 have been devised, but their performance needs to be validated in comparison with quantitative immunoassays. First, using 1749 PRNT-positive sera, we noticed that log-transformed optical density (OD) ratio of wild-type (WT) sVNT exhibited better titer-correlation with plaque reduction neutralization test (PRNT) than % inhibition value. Second, we tried 798 dilutional titration tests with 103 sera, but nonlinear correlation between OD ratio and antibody concentration limited titration of sVNT. Third, the titer-correlations of two sVNT kits for BA.1 and two quantitative immunoassays for WT were evaluated with BA.1 and BA.5 PRNT. All tested kits exhibited a linear correlation with PRNT titers, but the sVNT kits exhibited high false-negative rates (cPass-BA.1 kit, 45.4% for BA.1 and 44.2% for BA.5; STANDARD F-BA.1 kit, 1.9% for BA.1 and 2.2% for BA.5), while quantitative immunoassays showed 100% sensitivity. Linear mixed-effects model suggested superior titer-correlation with PRNT for quantitative immunoassays compared to sVNT kits. Taken together, the use of quantitative immunoassays for WT, rather than rapid development of new kits, would be practical for predicting neutralizing activities against emerging new variants.
Full Text
https://onlinelibrary.wiley.com/doi/10.1002/jmv.29329
DOI
10.1002/jmv.29329
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yong Chan(김용찬)
Park, Yoon Soo(박윤수)
Song, Young Goo(송영구) ORCID logo https://orcid.org/0000-0002-0733-4156
Ahn, Jin Young(안진영) ORCID logo https://orcid.org/0000-0002-3740-2826
Lee, Kyoung Hwa(이경화) ORCID logo https://orcid.org/0000-0003-0033-1398
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/197791
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