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B cell responses to membrane-presented antigens require the function of the mechanosensitive cation channel Piezo1

Authors
 Kihyuck Kwak  ;  Haewon Sohn  ;  Rachel George  ;  Charles Torgbor  ;  Javier Manzella-Lapeira  ;  Joseph Brzostowski  ;  Susan K Pierce 
Citation
 SCIENCE SIGNALING, Vol.16(804) : eabq5096, 2023-09 
Journal Title
SCIENCE SIGNALING
ISSN
 1937-9145 
Issue Date
2023-09
MeSH
B-Lymphocytes ; COVID-19* ; Cations ; Cell Membrane ; Humans ; Lymphocyte Activation
Abstract
The demand for a vaccine for coronavirus disease 2019 (COVID-19) highlighted gaps in our understanding of the requirements for B cell responses to antigens, particularly to membrane-presented antigens, as occurs in vivo. We found that human B cell responses to membrane-presented antigens required the function of Piezo1, a plasma membrane mechanosensitive cation channel. Simply making contact with a glass probe induced calcium (Ca2+) fluxes in B cells that were blocked by the Piezo1 inhibitor GsMTx4. When placed on glass surfaces, the plasma membrane tension of B cells increased, which stimulated Ca2+ influx and spreading of B cells over the glass surface, which was blocked by the Piezo1 inhibitor OB-1. B cell responses to membrane-presented antigens but not to soluble antigens were inhibited both by Piezo1 inhibitors and by siRNA-mediated knockdown of Piezo1. Thus, the activation of Piezo1 defines an essential event in B cell activation to membrane-presented antigens that may be exploited to improve the efficacy of vaccines.
Files in This Item:
T202307395.pdf Download
DOI
10.1126/scisignal.abq5096
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Kihyuck(곽기혁)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/197736
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