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O2 variant chip to simulate site-specific skeletogenesis from hypoxic bone marrow

Authors
 Hye-Seon Kim  ;  Hyun-Su Ha  ;  Dae-Hyun Kim  ;  Deok Hyeon Son  ;  Sewoom Baek  ;  Jeongeun Park  ;  Chan Hee Lee  ;  Suji Park  ;  Hyo-Jin Yoon  ;  Seung Eun Yu  ;  Jeon Il Kang  ;  Kyung Min Park  ;  Young Min Shin  ;  Jung Bok Lee  ;  Hak-Joon Sung 
Citation
 SCIENCE ADVANCES, Vol.9(12) : eadd4210, 2023-03 
Journal Title
SCIENCE ADVANCES
Issue Date
2023-03
MeSH
Animals ; Bone Marrow Cells ; Bone Marrow* ; Cartilage* ; Cell Differentiation ; Cells, Cultured ; Hypoxia ; Osteogenesis ; Oxygen ; Rabbits
Abstract
The stemness of bone marrow mesenchymal stem cells (BMSCs) is maintained by hypoxia. The oxygen level increases from vessel-free cartilage to hypoxic bone marrow and, furthermore, to vascularized bone, which might direct the chondrogenesis to osteogenesis and regenerate the skeletal system. Hence, oxygen was diffused from relatively low to high levels throughout a three-dimensional chip. When we cultured BMSCs in the chip and implanted them into the rabbit defect models of low-oxygen cartilage and high-oxygen calvaria bone, (i) the low oxygen level (base) promoted stemness and chondrogenesis of BMSCs with robust antioxidative potential; (ii) the middle level (two times ≥ low) pushed BMSCs to quiescence; and (iii) the high level (four times ≥ low) promoted osteogenesis by disturbing the redox balance and stemness. Last, endochondral or intramembranous osteogenesis upon transition from low to high oxygen in vivo suggests a developmental mechanism-driven solution to promote chondrogenesis to osteogenesis in the skeletal system by regulating the oxygen environment.
Files in This Item:
T202302934.pdf Download
DOI
10.1126/sciadv.add4210
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Sung, Hak-Joon(성학준) ORCID logo https://orcid.org/0000-0003-2312-2484
Shin, Young Min(신영민)
Yu, Seung Eun(유승은) ORCID logo https://orcid.org/0000-0002-9690-8739
Ha, Hyun-Su(하현수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/195318
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