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Serodiagnosis of human sparganosis by a monoclonal antibody-based competition ELISA

Authors
 In Sok Yeo  ;  Tai Soon Yong  ;  Kyung il Im 
Citation
 YONSEI MEDICAL JOURNAL, Vol.35(1) : 43-48, 1994-03 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
1994-03
MeSH
Animals ; Antibodies, Helminth ; Binding, Competitive ; Enzyme-Linked Immunosorbent Assay ; Humans ; Sensitivity and Specificity ; Sparganosis / diagnosis* ; Sparganum / immunology
Abstract
Competition ELISA test using sparganum specific monoclonal antibodies (Mab) was investigated to improve the diagnostic specificity of sparganosis. By cell fusion, one hybridoma clone secreting anti-sparganum specific Mab was selected (Sp-20), which reacted on bands of 32 kDa and 38 kDa. Sp-20 reacted on calcium corpuscles on IFA. By micro-ELISA, 16 of 17 sparganosis cases (95%) were found positive, but 1 of 18 clonorchiasis cases (5%), 4 of 16 cysticercosis cases (25%) and 2 of 16 normal controls (11%) showed false positive reactions. On the other hand, by competition ELISA using a sparganum specific Mab (Sp-20), 16 out of 17 (95%) of sparganosis cases were found positive, but 2 of 18 clonorchiasis cases (10%), 2 of 16 cysticercosis cases (12%), 3 of 16 paragonimiasis cases (18%) and 1 of 16 normal controls (6%) showed false positive reactions.
Files in This Item:
T199401408.pdf Download
DOI
10.3349/ymj.1994.35.1.43
Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/195148
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