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Biological reaction mediated engineered AuNPs facilitated delivery encore the anticancer, antiglycation, and antidiabetic potential of garcinol

Authors
 Zeeshan Rafi  ;  Mohammad Hassan Baig  ;  Fohad Mabood Husain  ;  Suliman Yousef Alomar  ;  Jae-June Dong  ;  Mohd Sajid Khan 
Citation
 JOURNAL OF KING SAUD UNIVERSITY SCIENCE, Vol.35(3) : 102524, 2023-04 
Journal Title
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
ISSN
 1018-3647 
Issue Date
2023-04
Keywords
Gold nanoparticles ; Lung cancer ; Glycation ; Garcinol ; α-amylase
Abstract
Objectives
Diabetes mellitus (DM), cancer and cardiovascular diseases (CVD) are major contributors to human miseries and death. Additionally, previous findings stated that cancer is also known to be associated with diabetes. Therefore, in the given study, facile synthesis of gold nanoparticles (AuNPs) using glycation reaction was achieved and further, their bioconjugation with herbal drug garcinol (G) was performed (G-AuNPs) in order to enhance the antiglycative, antidiabetic and anticancer efficacy of the garcinol.

Methods
The antiglycation and antidiabetic potential of garcinol bioconjugated AuNPs (G-AuNPs) were assessed through different experiments. Further, anticancer potential against adenocarcinoma cells were also assessed.

Results
The study confirmed 78.6 % bioconjugation of G over the surface of AuNPs. Furthermore, the antiglycation study depicted that G-AuNPs are found to be more effective inhibitor of the glycation reaction in contrast to pure G. The findings represented that G-AuNPs significantly blocks the formation of early glycation adducts and AGEs, also they mask the glycation prone free arginine and lysine residues from participating in the glycation process. Additionally, our α-amylase inhibition assay results demonstrated that pure G (IC50 − 8.9 µM) has significantly higher α-amylase inhibition activity compared to standard inhibitor, acarbose (IC50 − 0.118 mM). Also, the anticancer study described that G and G-AuNPs treatment resulted in death of the cells via apoptosis, which resulted in elevated permeability, condensed chromatin, deep blue fluorescent, and condensed nucleus of A549 cells. Our findings also revealed that the potential of mitochondrial membrane (ΔΨm) of A549 cells were also disrupted at much lower concentration of G-AuNPs (13.3 µM) as compared to pure G (28.7 µM).

Conclusion
The key findings of the investigation suggested that G-AuNPs acted as a potent antiglycation, antidiabetic, and anticancer agent.
Files in This Item:
T202300244.pdf Download
DOI
10.1016/j.jksus.2022.102524
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Family Medicine (가정의학교실) > 1. Journal Papers
Yonsei Authors
Dong, Jae June(동재준) ORCID logo https://orcid.org/0000-0002-2420-2155
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/193520
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