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Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis

 Nahee Hwang  ;  Yune Huh  ;  Seonghyeon Bu  ;  Kyung Jin Seo  ;  Se Hwan Kwon  ;  Jae-Woo Kim  ;  Bo Kyung Yoon  ;  Hyo-Suk Ahn  ;  Sungsoon Fang 
 FRONTIERS IN IMMUNOLOGY, Vol.13 : 979188, 2022-09 
Journal Title
Issue Date
BNT162 Vaccine ; COVID-19* ; Fatty Acids ; Humans ; Leukocytes, Mononuclear ; Monocytes ; Myocarditis* / genetics ; SARS-CoV-2 ; Transcription Factor AP-1 ; Transcriptome ; Vaccines, Synthetic ; mRNA Vaccines
BNT162b2 ; Coronavirus - COVID-19 ; monocyte - macrophage ; myocarditis ; single-cell RNA sequencing ; transcriptome (RNA-seq) ; vaccination
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been the most dangerous threat to public health worldwide for the last few years, which led to the development of the novel mRNA vaccine (BNT162b2). However, BNT162b2 vaccination is known to be associated with myocarditis. Here, as an attempt to determine the pathogenesis of the disease and to develop biomarkers to determine whether subjects likely proceed to myocarditis after vaccination, we conducted a time series analysis of peripheral blood mononuclear cells of a patient with BNT162b2-induced myocarditis. Single-cell RNA sequence analysis identified monocytes as the cell clusters with the most dynamic changes. To identify distinct gene expression signatures, we compared monocytes of BNT162b2-induced myocarditis with monocytes under various conditions, including SARS-CoV-2 infection, BNT162b2 vaccination, and Kawasaki disease, a disease similar to myocarditis. Representative changes in the transcriptomic profile of classical monocytes include the upregulation of genes related to fatty acid metabolism and downregulation of transcription factor AP-1 activity. This study provides, for the first time, the importance of classical monocytes in the pathogenesis of myocarditis following BNT162b2 vaccination and presents the possibility that vaccination affects monocytes, further inducing their differentiation and infiltration into the heart.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
Yoon, Bo Kyung(윤보경)
Fang, Sungsoon(황성순) ORCID logo https://orcid.org/0000-0003-0201-5567
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