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Development of Cryopreserved Red Blood Cell Panels as Biological Reference Standards for Performance Evaluation of ABO and D Blood Grouping Reagents

Other Titles
 ABO 및 D형 혈액형 판정용 동결 적혈구 국가표준품 제조 및 확립 
Authors
 Youn Keong Cho  ;  Sinyoung Kim  ;  Jihyang Lim  ;  Dae-Hyun Ko  ;  Seung Jun Choi  ;  Hyun Ok Kim 
Citation
 Laboratory Medicine Online, Vol.12(1) : 1-10, 2022-01 
Journal Title
Laboratory Medicine Online
Issue Date
2022-01
Keywords
Cryopreservation ; Red blood cells ; Blood grouping reagent ; High-glycerol method
Abstract
Background: Accurate blood typing is essential for blood transfusions, and requires the constant evaluation and maintenance of ABO and D blood grouping reagents. In the present study, we developed cryopreserved red blood cell (RBC) panels and evaluated their feasibility as a standard reference material to verify the quality of ABO and D blood grouping reagents in Korea.
Methods: RBC units obtained from healthy donors were cryopreserved using a high-glycerol method. A total of 400 sets of RBC panels were prepared, composed of blood group A (N=5), B (N=5), O (N=10), AB (N=4), Rh D-positive (N=4), Rh D-negative (N=5), and weak-D (N=1), and 200 sets of RBC subgroup panels composed of A2, A2B, A2B3, A1B3, and B3, and A2, A2B, A2B3, A1B3, and A3B (N=1, each). Quality assessment of the cryopreserved RBC panels before and after cryopreservation was performed by measuring their sensitivity, specificity, avidity, and potency titers.
Results: Our cryopreserved ABO and D RBC panels had a sensitivity and specificity of 100% to existing monoclonal blood grouping reagents, regardless of blood type and cryopreservation time. There were no significant differences in the avidity time and potency titers of the cryopreserved RBCs before and after 6 or 12 months of cryopreservation.
Conclusions: The quality parameters measured here suggest that our newly developed cryopreserved RBC panels were reliable for use as a standard reference material for the performance evaluation of anti-A, -B, and -D blood grouping reagents.
Files in This Item:
T202205133.pdf Download
DOI
10.47429/lmo.2022.12.1.1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Cho, Youn Keong(조윤경) ORCID logo https://orcid.org/0000-0002-7922-0473
Choi, Seung Jun(최승준) ORCID logo https://orcid.org/0000-0003-0736-1055
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/191164
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