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Isolation and characterization of dental follicle-derived Hertwig's epithelial root sheath cells

Authors
 Ju Eun Oh  ;  Jin-Kyu Yi 
Citation
 CLINICAL ORAL INVESTIGATIONS, Vol.25(4) : 1787-1796, 2021-04 
Journal Title
CLINICAL ORAL INVESTIGATIONS
ISSN
 1432-6981 
Issue Date
2021-04
MeSH
Cell Differentiation ; Dental Cementum* ; Dental Sac* ; Epithelial Cells ; Humans ; Osteogenesis ; Tooth Root
Keywords
Cementogenesis ; Dental follicle–derived HERS cells ; HERSC-dMSC interactions ; Regenerative endodontic procedures
Abstract
Objectives: The aim of this study was the isolation and characterization of dental follicle-derived Hertwig's epithelial root sheath cells (DF-HERSCs).

Materials and methods: DF-HERSCs were isolated from dental follicle (DF)-derived single-cell suspensions. Their epithelial phenotypes were analyzed by Western blotting, polymerase chain reaction (PCR), and quantitative polymerase chain reaction (qPCR). Epithelial-mesenchymal transition (EMT) was induced in DF-HERSCs by treatment with transforming growth factor-β (TGF-β) or fetal bovine serum (FBS)-added medium. Characteristics of DF-HERSCs were compared with normal human oral keratinocytes (NHOKs) and normal human epidermal keratinocytes (NHEKs). Osteogenic differentiation and mineralization of DF-HERSCs were analyzed by alkaline phosphatase (ALP) and Alizarin red staining. All experiments were conducted in triplicate.

Results: Primary DF-HERSCs were isolated from DF. Epithelial phenotypes of DF-HERSCs were confirmed by morphological and Western blot analysis. PCR results demonstrated that the origin of DF-HERSCs was neither endothelial nor hematopoietic. Enamel matrix derivative (EMD)-associated genes were not expressed in DF-HERSCs. Treatment with TGF-β and FBS-added medium triggered the progression of EMT in DF-HERSCs. The acquired potency of differentiation and mineralization was shown in EMT-progressed DF-HERSCs.

Conclusions: DF contains putative populations of HERSC, named DF-HERSC. DF-HERSCs shared common characteristics with NHOKs and NHEKs.
Full Text
https://link.springer.com/article/10.1007/s00784-020-03481-4
DOI
10.1007/s00784-020-03481-4
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Oh, Ju Eun(오주은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190980
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