Cited 3 times in
Isolation and characterization of dental follicle-derived Hertwig's epithelial root sheath cells
DC Field | Value | Language |
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dc.contributor.author | 오주은 | - |
dc.date.accessioned | 2022-11-24T00:47:25Z | - |
dc.date.available | 2022-11-24T00:47:25Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 1432-6981 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/190980 | - |
dc.description.abstract | Objectives: The aim of this study was the isolation and characterization of dental follicle-derived Hertwig's epithelial root sheath cells (DF-HERSCs). Materials and methods: DF-HERSCs were isolated from dental follicle (DF)-derived single-cell suspensions. Their epithelial phenotypes were analyzed by Western blotting, polymerase chain reaction (PCR), and quantitative polymerase chain reaction (qPCR). Epithelial-mesenchymal transition (EMT) was induced in DF-HERSCs by treatment with transforming growth factor-β (TGF-β) or fetal bovine serum (FBS)-added medium. Characteristics of DF-HERSCs were compared with normal human oral keratinocytes (NHOKs) and normal human epidermal keratinocytes (NHEKs). Osteogenic differentiation and mineralization of DF-HERSCs were analyzed by alkaline phosphatase (ALP) and Alizarin red staining. All experiments were conducted in triplicate. Results: Primary DF-HERSCs were isolated from DF. Epithelial phenotypes of DF-HERSCs were confirmed by morphological and Western blot analysis. PCR results demonstrated that the origin of DF-HERSCs was neither endothelial nor hematopoietic. Enamel matrix derivative (EMD)-associated genes were not expressed in DF-HERSCs. Treatment with TGF-β and FBS-added medium triggered the progression of EMT in DF-HERSCs. The acquired potency of differentiation and mineralization was shown in EMT-progressed DF-HERSCs. Conclusions: DF contains putative populations of HERSC, named DF-HERSC. DF-HERSCs shared common characteristics with NHOKs and NHEKs. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Springer-Verlag | - |
dc.relation.isPartOf | CLINICAL ORAL INVESTIGATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Dental Cementum* | - |
dc.subject.MESH | Dental Sac* | - |
dc.subject.MESH | Epithelial Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Osteogenesis | - |
dc.subject.MESH | Tooth Root | - |
dc.title | Isolation and characterization of dental follicle-derived Hertwig's epithelial root sheath cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Research Institute (부설연구소) | - |
dc.contributor.googleauthor | Ju Eun Oh | - |
dc.contributor.googleauthor | Jin-Kyu Yi | - |
dc.identifier.doi | 10.1007/s00784-020-03481-4 | - |
dc.contributor.localId | A05853 | - |
dc.relation.journalcode | J00601 | - |
dc.identifier.eissn | 1436-3771 | - |
dc.identifier.pmid | 32749551 | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00784-020-03481-4 | - |
dc.subject.keyword | Cementogenesis | - |
dc.subject.keyword | Dental follicle–derived HERS cells | - |
dc.subject.keyword | HERSC-dMSC interactions | - |
dc.subject.keyword | Regenerative endodontic procedures | - |
dc.contributor.alternativeName | Oh, Ju Eun | - |
dc.contributor.affiliatedAuthor | 오주은 | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1787 | - |
dc.citation.endPage | 1796 | - |
dc.identifier.bibliographicCitation | CLINICAL ORAL INVESTIGATIONS, Vol.25(4) : 1787-1796, 2021-04 | - |
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