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Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas

 Nanda Maya Mali  ;  Jung Min Park  ;  Gil-Hyun Kim  ;  Dong-Hwa Choi  ;  Raul Ramos  ;  Jong Hyuk Lee  ;  Eu Jeong Ku  ;  Won Oh 
 Anatomy & Biological Anthropology (해부·생물인류학), Vol.35(2) : 57-66, 2022-06 
Journal Title
Anatomy & Biological Anthropology(해부·생물인류학)
Issue Date
Light-sheet fluorescence microscopy ; Pancreas ; Three-dimensional ; Tissue clearing ; Vascular system
Optical imaging of the vascular system is necessary for structural, functional, developmental, and
pathological biology. Recent developments in optical tissue-clearing methods have significantly advanced large scale tissue researches by integrating a three-dimensional (3D) visualization of topographical vasculature, so we
aimed to enhance the vascular structure of the murine pancreas which are highly vascularized structure. In this
study, the adult C57/BL6 murine pancreatic tissue was cleared after lectin staining, resulting in the reconstructed
structures for 3D imaging within a short period without damaging. Enhancing the visualization processes using
light-sheet fluorescence microscopy (LSFM) together with 3D reconstruction imaging tool, we studied in depth
of the entire vascular system, which further enabled to reconstruct 3D structure of the vasculature in pancreas
and islets. We successfully implemented the normal murine pancreatic tissues including exocrine and endocrine cells and vascular structures, reflecting pancreatic β-cell function. The size of islets of each pancreatic lobe were
not different but the number of islets in each lobe was variable in our quantification. In conclusion, this deep
tissue clearing technique for 3D imaging could be applied in future studies to further understand the topological
interactions of various cells and blood vessels within intact organs and tissues.
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1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Oh, Ji Won(오지원) ORCID logo https://orcid.org/0000-0001-5742-5120
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