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Allergenic characterization of Bomb m 4, a 30-kDa Bombyx mori lipoprotein 6 from silkworm pupa

Authors
 Kyoung Yong Jeong  ;  Jong Sun Lee  ;  Ji Eun Yuk  ;  Hangyeol Song  ;  Hye Jung Lee  ;  Kuan Jung Kim  ;  Bum Joon Kim  ;  Kook-Jin Lim  ;  Kyung Hee Park  ;  Jae-Hyun Lee  ;  Jung-Won Park 
Citation
 CLINICAL AND EXPERIMENTAL ALLERGY, Vol.52(7) : 888-897, 2022-07 
Journal Title
CLINICAL AND EXPERIMENTAL ALLERGY
ISSN
 0954-7894 
Issue Date
2022-07
MeSH
Allergens* ; Animals ; Bombyx* ; Cross Reactions ; Epitopes ; Glycoproteins ; Humans ; Hypersensitivity* ; Immunoglobulin E ; Insect Proteins* / immunology ; Lipoproteins ; Proteomics ; Pupa ; Recombinant Proteins
Keywords
food allergy ; major allergen ; silkworm pupa allergy
Abstract
Background: Silkworm pupa (SWP) food anaphylaxis has been described frequently in Asian countries. However, false-positive reactions by skin pricks and serum IgE (sIgE) tests to the extract complicate diagnosis, requiring identification of clinically relevant major allergens.

Objectives: In this study, we characterized a novel SWP allergen, Bomb m 4, a 30-kDa lipoprotein, and evaluated its diagnostic sensitivity.

Methods: Bomb m 4 was identified by a proteomic analysis. This recombinant (r)Bomb m 4 was overexpressed in Escherichia coli, and the IgE reactivity by ELISA was compared with other reported allergenic proteins: Bomb m 1 (arginine kinase), 27-kDa glycoprotein, Bomb m 3 (tropomyosin) using the serum samples from 17 SWP allergic patients and 11 asymptomatic sensitized subjects.

Results: rBomb m 4-specific IgE was recognized by all 17 SWP allergic patients. The 27-kDa glycoprotein and Bomb m 1 sIgE were found in 35.3% and 0%, respectively, in the SWP allergic patients. ELISA sIgE reactivity increased significantly, when 4 M urea was added in serum samples. However, only 16% inhibition of sIgE reactivity to the whole SWP extract was exhibited by rBomb m 4, whereas more than 93% of self-inhibition of rBomb m 4 sIgE was obtained, possibly due to the low abundance of Bomb m 4 in the extract. Three linear epitopes (81-95, 191-205 and 224-238 residues) of rBomb m 4 were identified. These epitopes are shown to be released by pepsin digestion. Receiver operator characteristic (ROC) analysis showed the highest diagnostic value of Bomb m 4 followed by Bomb m 1, 27-kDa glycoprotein and Bomb m 3.

Conclusion: Bomb m 4 is the major allergen of SWP allergic patients. It has cryptic epitopes which are exposed to IgE antibodies with digestive enzymes. This recombinant Bomb m 4 allergen permits exact diagnosis of SWP allergy.
Full Text
https://onlinelibrary.wiley.com/doi/10.1111/cea.14095
DOI
10.1111/cea.14095
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Hee(박경희) ORCID logo https://orcid.org/0000-0003-3605-5364
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189450
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