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Allergenic characterization of Bomb m 4, a 30-kDa Bombyx mori lipoprotein 6 from silkworm pupa
DC Field | Value | Language |
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dc.contributor.author | 박경희 | - |
dc.contributor.author | 박중원 | - |
dc.contributor.author | 이재현 | - |
dc.contributor.author | 정경용 | - |
dc.date.accessioned | 2022-08-23T00:28:35Z | - |
dc.date.available | 2022-08-23T00:28:35Z | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 0954-7894 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/189450 | - |
dc.description.abstract | Background: Silkworm pupa (SWP) food anaphylaxis has been described frequently in Asian countries. However, false-positive reactions by skin pricks and serum IgE (sIgE) tests to the extract complicate diagnosis, requiring identification of clinically relevant major allergens. Objectives: In this study, we characterized a novel SWP allergen, Bomb m 4, a 30-kDa lipoprotein, and evaluated its diagnostic sensitivity. Methods: Bomb m 4 was identified by a proteomic analysis. This recombinant (r)Bomb m 4 was overexpressed in Escherichia coli, and the IgE reactivity by ELISA was compared with other reported allergenic proteins: Bomb m 1 (arginine kinase), 27-kDa glycoprotein, Bomb m 3 (tropomyosin) using the serum samples from 17 SWP allergic patients and 11 asymptomatic sensitized subjects. Results: rBomb m 4-specific IgE was recognized by all 17 SWP allergic patients. The 27-kDa glycoprotein and Bomb m 1 sIgE were found in 35.3% and 0%, respectively, in the SWP allergic patients. ELISA sIgE reactivity increased significantly, when 4 M urea was added in serum samples. However, only 16% inhibition of sIgE reactivity to the whole SWP extract was exhibited by rBomb m 4, whereas more than 93% of self-inhibition of rBomb m 4 sIgE was obtained, possibly due to the low abundance of Bomb m 4 in the extract. Three linear epitopes (81-95, 191-205 and 224-238 residues) of rBomb m 4 were identified. These epitopes are shown to be released by pepsin digestion. Receiver operator characteristic (ROC) analysis showed the highest diagnostic value of Bomb m 4 followed by Bomb m 1, 27-kDa glycoprotein and Bomb m 3. Conclusion: Bomb m 4 is the major allergen of SWP allergic patients. It has cryptic epitopes which are exposed to IgE antibodies with digestive enzymes. This recombinant Bomb m 4 allergen permits exact diagnosis of SWP allergy. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Blackwell Scientific Publications | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL ALLERGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Allergens* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bombyx* | - |
dc.subject.MESH | Cross Reactions | - |
dc.subject.MESH | Epitopes | - |
dc.subject.MESH | Glycoproteins | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity* | - |
dc.subject.MESH | Immunoglobulin E | - |
dc.subject.MESH | Insect Proteins* / immunology | - |
dc.subject.MESH | Lipoproteins | - |
dc.subject.MESH | Proteomics | - |
dc.subject.MESH | Pupa | - |
dc.subject.MESH | Recombinant Proteins | - |
dc.title | Allergenic characterization of Bomb m 4, a 30-kDa Bombyx mori lipoprotein 6 from silkworm pupa | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Jong Sun Lee | - |
dc.contributor.googleauthor | Ji Eun Yuk | - |
dc.contributor.googleauthor | Hangyeol Song | - |
dc.contributor.googleauthor | Hye Jung Lee | - |
dc.contributor.googleauthor | Kuan Jung Kim | - |
dc.contributor.googleauthor | Bum Joon Kim | - |
dc.contributor.googleauthor | Kook-Jin Lim | - |
dc.contributor.googleauthor | Kyung Hee Park | - |
dc.contributor.googleauthor | Jae-Hyun Lee | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.identifier.doi | 10.1111/cea.14095 | - |
dc.contributor.localId | A01427 | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A03086 | - |
dc.contributor.localId | A03572 | - |
dc.relation.journalcode | J00548 | - |
dc.identifier.eissn | 1365-2222 | - |
dc.identifier.pmid | 35028994 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/cea.14095 | - |
dc.subject.keyword | food allergy | - |
dc.subject.keyword | major allergen | - |
dc.subject.keyword | silkworm pupa allergy | - |
dc.contributor.alternativeName | Park, Kyung Hee | - |
dc.contributor.affiliatedAuthor | 박경희 | - |
dc.contributor.affiliatedAuthor | 박중원 | - |
dc.contributor.affiliatedAuthor | 이재현 | - |
dc.contributor.affiliatedAuthor | 정경용 | - |
dc.citation.volume | 52 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 888 | - |
dc.citation.endPage | 897 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL ALLERGY, Vol.52(7) : 888-897, 2022-07 | - |
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