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Alterations in phospholipid profiles of erythrocytes deep-frozen without cryoprotectants

Authors
 Yoeseph Cho  ;  Ji-Hye Woo  ;  Oh-Seung Kwon  ;  Sang Sun Yoon  ;  Junghyun Son 
Citation
 DRUG TESTING AND ANALYSIS, Vol.11(8) : 1231-1237, 2019-08 
Journal Title
DRUG TESTING AND ANALYSIS
ISSN
 1942-7603 
Issue Date
2019-08
MeSH
Blood Preservation / methods* ; Cryopreservation / methods* ; Erythrocyte Membrane / chemistry ; Erythrocytes / chemistry* ; Erythrocytes / cytology ; Humans ; Phospholipids / analysis* ; Phosphorylcholine / analogs & derivatives ; Phosphorylcholine / analysis ; Sphingosine / analogs & derivatives ; Sphingosine / analysis
Keywords
autologous blood transfusion ; erythrocytes ; liquid chromatography ; mass spectrometry ; phospholipid profiles
Abstract
The erythrocyte membrane is composed of a phospholipid bilayer, which is known to undergo physicochemical changes during storage at low temperatures. This study was conducted to identify marker phospholipids that indicate alteration during deep-frozen storage and to determine the amount of marker phospholipids. Our research suggested a method to detect phospholipids by profiling analysis of thermally injured red blood cells (RBCs) without protecting agents. Human blood was stored at -80 degrees C for 72 days. The RBC membrane phospholipids were extracted through a modified Bligh and Dyer method. Six selected phospholipids were analyzed and quantified using liquid chromatography-tandem mass spectrometry, and an in vitro model system was developed. The intracellular level of N-nervonoyl-D-erythro-sphingosylphosphorylcholine significantly increased in the thermally injured RBCs, and multiple biomarker candidates were evaluated by profiling analysis and mass spectrometry technology for targeted metabolomics.
Full Text
https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/dta.2600
DOI
10.1002/dta.2600
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/189137
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