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Alterations in phospholipid profiles of erythrocytes deep-frozen without cryoprotectants

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dc.contributor.author윤상선-
dc.date.accessioned2022-08-19T06:24:15Z-
dc.date.available2022-08-19T06:24:15Z-
dc.date.issued2019-08-
dc.identifier.issn1942-7603-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/189137-
dc.description.abstractThe erythrocyte membrane is composed of a phospholipid bilayer, which is known to undergo physicochemical changes during storage at low temperatures. This study was conducted to identify marker phospholipids that indicate alteration during deep-frozen storage and to determine the amount of marker phospholipids. Our research suggested a method to detect phospholipids by profiling analysis of thermally injured red blood cells (RBCs) without protecting agents. Human blood was stored at -80 degrees C for 72 days. The RBC membrane phospholipids were extracted through a modified Bligh and Dyer method. Six selected phospholipids were analyzed and quantified using liquid chromatography-tandem mass spectrometry, and an in vitro model system was developed. The intracellular level of N-nervonoyl-D-erythro-sphingosylphosphorylcholine significantly increased in the thermally injured RBCs, and multiple biomarker candidates were evaluated by profiling analysis and mass spectrometry technology for targeted metabolomics.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherJohn Wiley & Sons-
dc.relation.isPartOfDRUG TESTING AND ANALYSIS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHBlood Preservation / methods*-
dc.subject.MESHCryopreservation / methods*-
dc.subject.MESHErythrocyte Membrane / chemistry-
dc.subject.MESHErythrocytes / chemistry*-
dc.subject.MESHErythrocytes / cytology-
dc.subject.MESHHumans-
dc.subject.MESHPhospholipids / analysis*-
dc.subject.MESHPhosphorylcholine / analogs & derivatives-
dc.subject.MESHPhosphorylcholine / analysis-
dc.subject.MESHSphingosine / analogs & derivatives-
dc.subject.MESHSphingosine / analysis-
dc.titleAlterations in phospholipid profiles of erythrocytes deep-frozen without cryoprotectants-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학교실)-
dc.contributor.googleauthorYoeseph Cho-
dc.contributor.googleauthorJi-Hye Woo-
dc.contributor.googleauthorOh-Seung Kwon-
dc.contributor.googleauthorSang Sun Yoon-
dc.contributor.googleauthorJunghyun Son-
dc.identifier.doi10.1002/dta.2600-
dc.contributor.localIdA02558-
dc.relation.journalcodeJ04232-
dc.identifier.eissn1942-7611-
dc.identifier.pmid30950199-
dc.identifier.urlhttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/dta.2600-
dc.subject.keywordautologous blood transfusion-
dc.subject.keyworderythrocytes-
dc.subject.keywordliquid chromatography-
dc.subject.keywordmass spectrometry-
dc.subject.keywordphospholipid profiles-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.affiliatedAuthor윤상선-
dc.citation.volume11-
dc.citation.number8-
dc.citation.startPage1231-
dc.citation.endPage1237-
dc.identifier.bibliographicCitationDRUG TESTING AND ANALYSIS, Vol.11(8) : 1231-1237, 2019-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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