Cited 3 times in
Alterations in phospholipid profiles of erythrocytes deep-frozen without cryoprotectants
DC Field | Value | Language |
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dc.contributor.author | 윤상선 | - |
dc.date.accessioned | 2022-08-19T06:24:15Z | - |
dc.date.available | 2022-08-19T06:24:15Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.issn | 1942-7603 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/189137 | - |
dc.description.abstract | The erythrocyte membrane is composed of a phospholipid bilayer, which is known to undergo physicochemical changes during storage at low temperatures. This study was conducted to identify marker phospholipids that indicate alteration during deep-frozen storage and to determine the amount of marker phospholipids. Our research suggested a method to detect phospholipids by profiling analysis of thermally injured red blood cells (RBCs) without protecting agents. Human blood was stored at -80 degrees C for 72 days. The RBC membrane phospholipids were extracted through a modified Bligh and Dyer method. Six selected phospholipids were analyzed and quantified using liquid chromatography-tandem mass spectrometry, and an in vitro model system was developed. The intracellular level of N-nervonoyl-D-erythro-sphingosylphosphorylcholine significantly increased in the thermally injured RBCs, and multiple biomarker candidates were evaluated by profiling analysis and mass spectrometry technology for targeted metabolomics. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | John Wiley & Sons | - |
dc.relation.isPartOf | DRUG TESTING AND ANALYSIS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Blood Preservation / methods* | - |
dc.subject.MESH | Cryopreservation / methods* | - |
dc.subject.MESH | Erythrocyte Membrane / chemistry | - |
dc.subject.MESH | Erythrocytes / chemistry* | - |
dc.subject.MESH | Erythrocytes / cytology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Phospholipids / analysis* | - |
dc.subject.MESH | Phosphorylcholine / analogs & derivatives | - |
dc.subject.MESH | Phosphorylcholine / analysis | - |
dc.subject.MESH | Sphingosine / analogs & derivatives | - |
dc.subject.MESH | Sphingosine / analysis | - |
dc.title | Alterations in phospholipid profiles of erythrocytes deep-frozen without cryoprotectants | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Yoeseph Cho | - |
dc.contributor.googleauthor | Ji-Hye Woo | - |
dc.contributor.googleauthor | Oh-Seung Kwon | - |
dc.contributor.googleauthor | Sang Sun Yoon | - |
dc.contributor.googleauthor | Junghyun Son | - |
dc.identifier.doi | 10.1002/dta.2600 | - |
dc.contributor.localId | A02558 | - |
dc.relation.journalcode | J04232 | - |
dc.identifier.eissn | 1942-7611 | - |
dc.identifier.pmid | 30950199 | - |
dc.identifier.url | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/dta.2600 | - |
dc.subject.keyword | autologous blood transfusion | - |
dc.subject.keyword | erythrocytes | - |
dc.subject.keyword | liquid chromatography | - |
dc.subject.keyword | mass spectrometry | - |
dc.subject.keyword | phospholipid profiles | - |
dc.contributor.alternativeName | Yoon, Sang Sun | - |
dc.contributor.affiliatedAuthor | 윤상선 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1231 | - |
dc.citation.endPage | 1237 | - |
dc.identifier.bibliographicCitation | DRUG TESTING AND ANALYSIS, Vol.11(8) : 1231-1237, 2019-08 | - |
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