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Comparative gene expression profiles of dental follicle at different stages of periodontal development: Combined use of laser capture microdissection and microarray

Authors
 Ping Dang  ;  Qinghuang Tang  ;  Min-yuan Nie  ;  Yu An  ;  Rui Dong  ;  Xu-dong Hua  ;  Han-Sung Jung  ;  Sheng-gen Shi 
Citation
 JOURNAL OF ORAL BIOSCIENCES, Vol.60(4) : 92-97, 2018-12 
Journal Title
JOURNAL OF ORAL BIOSCIENCES
ISSN
 1349-0079 
Issue Date
2018-12
MeSH
Laser capture microdissection (LCM) ; Microarray Gene expression profile ; Dental follicle ; Periodontal development
Abstract
Objectives
Odontogenesis is dependent on serial temporal and spatial epithelial-mesenchymal interaction. Multiple signaling networks have been identified in the inductive interactions of odontogenesis at the early stage. Technical obstacles and heterogeneity of the dental follicle (DF), which is the origin of the majority of periodontal tissues, have hindered the clarification of the molecular blueprint in early periodontal tissue development. This has in turn hindered studies of new/effective periodontal regeneration therapy. In this study, we comparatively analyzed the gene expression profiles of DF at E17 (when DF cells are histologically recognized) and PN2 (the initiation of periodontal development).

Methods
Gene expression profiles of DF at E17 and PN2 were assessed by the combined use of laser capture microdissection and microarray.

Results
Comparative gene expression analysis of DF at E17 and PN2 during periodontal development revealed > 2-fold up-regulation and down-regulation of 2519 and 5060 genes, respectively. Bioinformatic analysis of the selected genes revealed that the temporally changed genes were mostly enriched in GO terms relative to the vasculature system, and were sometimes linked to multiple processes. RT-qPCR was used to verify the microarray data.

Conclusions
The delineation of the differential gene expressions between pre- and post-natal developmental stages of DF in vivo will increase the understanding of periodontal tissue development.
Full Text
https://www.sciencedirect.com/science/article/pii/S1349007918300380
DOI
10.1016/j.job.2018.02.003
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/188870
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