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Microbiome and mycobiome interaction in house dust mites and impact on airway cells

 Ju Yeong Kim  ;  Myung-Hee Yi  ;  Seogwon Lee  ;  In-Yong Lee  ;  Dongeun Yong  ;  Sang Sun Yoon  ;  Tai-Soon Yong 
 CLINICAL AND EXPERIMENTAL ALLERGY, Vol.51(12) : 1592-1602, 2021-12 
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Background: Major allergen sources Dermatophagoides farinae, Dermatophagoides pteronyssinus and Tyrophagus putrescentiae have been reported to have distinct microbiomes. The purpose of this study was to investigate the effect of each mite on airway epithelial cells as a model of airway allergic disease.

Methods: Transcriptomic analysis (RNA-seq) of an airway epithelial cell line (BEAS-2B) was performed to compare gene expression patterns after treatment with extracts of three mite species (D. farinae, D. pteronyssinus and T. putrescentiae). In addition, mycobiome deep sequencing of mites was employed to identify fungal species that interact with the microbiomes of the mites.

Results: Immune responses to bacteria were enriched only in the D. farinae-treated group as this species harboured larger numbers of bacteria than the other mites, and the high level of LPS in D. farinae caused proinflammatory cytokine production in airway epithelial cells. In addition, antibiotic metabolism pathways were enriched in D. pteronyssinus-treated cells but not in D. farinae -treated cells. Subsequent experiments revealed that D. pteronyssinus had a high fungal load that inhibited bacterial survival in this mite species.

Conclusion: The large amount of bacteria in D. farinae causes airway epithelial cells to produce more allergy-related cytokines than D. pteronyssinus, since fungi present in D. pteronyssinus suppress the abundance of mite-associated bacteria.
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1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Young(김주영) ORCID logo https://orcid.org/0000-0003-2456-6298
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Lee, In Yong(이인용)
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