0 111

Cited 0 times in

Microbiome and mycobiome interaction in house dust mites and impact on airway cells

DC Field Value Language
dc.contributor.author김주영-
dc.contributor.author용동은-
dc.contributor.author용태순-
dc.contributor.author윤상선-
dc.contributor.author이명희-
dc.contributor.author이인용-
dc.date.accessioned2022-02-23T01:33:11Z-
dc.date.available2022-02-23T01:33:11Z-
dc.date.issued2021-12-
dc.identifier.issn0954-7894-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/187765-
dc.description.abstractBackground: Major allergen sources Dermatophagoides farinae, Dermatophagoides pteronyssinus and Tyrophagus putrescentiae have been reported to have distinct microbiomes. The purpose of this study was to investigate the effect of each mite on airway epithelial cells as a model of airway allergic disease. Methods: Transcriptomic analysis (RNA-seq) of an airway epithelial cell line (BEAS-2B) was performed to compare gene expression patterns after treatment with extracts of three mite species (D. farinae, D. pteronyssinus and T. putrescentiae). In addition, mycobiome deep sequencing of mites was employed to identify fungal species that interact with the microbiomes of the mites. Results: Immune responses to bacteria were enriched only in the D. farinae-treated group as this species harboured larger numbers of bacteria than the other mites, and the high level of LPS in D. farinae caused proinflammatory cytokine production in airway epithelial cells. In addition, antibiotic metabolism pathways were enriched in D. pteronyssinus-treated cells but not in D. farinae -treated cells. Subsequent experiments revealed that D. pteronyssinus had a high fungal load that inhibited bacterial survival in this mite species. Conclusion: The large amount of bacteria in D. farinae causes airway epithelial cells to produce more allergy-related cytokines than D. pteronyssinus, since fungi present in D. pteronyssinus suppress the abundance of mite-associated bacteria.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherBlackwell Scientific Publications-
dc.relation.isPartOfCLINICAL AND EXPERIMENTAL ALLERGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleMicrobiome and mycobiome interaction in house dust mites and impact on airway cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorJu Yeong Kim-
dc.contributor.googleauthorMyung-Hee Yi-
dc.contributor.googleauthorSeogwon Lee-
dc.contributor.googleauthorIn-Yong Lee-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorSang Sun Yoon-
dc.contributor.googleauthorTai-Soon Yong-
dc.identifier.doi10.1111/cea.13962-
dc.contributor.localIdA00937-
dc.contributor.localIdA02423-
dc.contributor.localIdA02424-
dc.contributor.localIdA02558-
dc.contributor.localIdA02761-
dc.relation.journalcodeJ00548-
dc.identifier.eissn1365-2222-
dc.identifier.pmid34087033-
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/10.1111/cea.13962-
dc.contributor.alternativeNameKim, Ju Young-
dc.contributor.affiliatedAuthor김주영-
dc.contributor.affiliatedAuthor용동은-
dc.contributor.affiliatedAuthor용태순-
dc.contributor.affiliatedAuthor윤상선-
dc.contributor.affiliatedAuthor이명희-
dc.citation.volume51-
dc.citation.number12-
dc.citation.startPage1592-
dc.citation.endPage1602-
dc.identifier.bibliographicCitationCLINICAL AND EXPERIMENTAL ALLERGY, Vol.51(12) : 1592-1602, 2021-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.