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Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

Authors
 Mi Hee Lee  ;  Ye Jin Park  ;  Seung Hee Hong  ;  Min-Ah Koo  ;  Minyoung Cho  ;  Jong-Chul Park 
Citation
 CELLS, Vol.10(11) : 2846, 2021-10 
Journal Title
CELLS(Cells)
ISSN
 2073-4409 
Issue Date
2021-10
Keywords
Golgi polarization ; adipose-derived stem cells ; directional migration ; electrotaxis
Abstract
Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.
Files in This Item:
T202105029.pdf Download
DOI
10.3390/cells10112846
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Koo, Min-Ah(구민아) ORCID logo https://orcid.org/0000-0002-8671-1131
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187091
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