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Cytokines in culture media of preimplantation embryos during in vitro fertilization: Impact on embryo quality

Authors
 Inha Lee  ;  So Hyun Ahn  ;  Hye In Kim  ;  Hye Won Baek  ;  Yun Jeong Park  ;  Heeyon Kim  ;  Aminah Ibrahim Aljassim  ;  Whan Shin  ;  Chungsoon Ryu  ;  Jeongmi Yoon  ;  Jae Hoon Lee  ;  Bo Hyon Yun  ;  Seok Kyo Seo  ;  Joo Hyun Park  ;  Young Sik Choi  ;  SiHyun Cho  ;  Byung Seok Lee 
Citation
 CYTOKINE, Vol.148 : 155714, 2021-12 
Journal Title
CYTOKINE
ISSN
 1043-4666 
Issue Date
2021-12
Keywords
CCL15 ; Cytokines ; Embryo culture medium ; Embryo quality ; IVF outcome
Abstract
Cytokine support of embryonic development includes promotion of implantation and protection of blastomeres from cell stress and apoptosis. Correlations between embryo quality and concentrations of specific cytokines in culture media of human embryos have been investigated for many years. The aim of this study was to assess the concentrations of cytokines in preimplantation embryo culture media and to investigate their relationships with embryo quality and in vitro fertilization (IVF) outcomes. Seventy-two samples were obtained from 39 infertile couples undergoing IVF or intracytoplasmic sperm injection treatment between October 2018 and May 2019. Each embryo was cultured separately, and the embryo culture medium was collected 72 h after fertilization. Before embryo transfer on day 3, a morphological evaluation of each embryo was performed. Cytokine concentrations of each culture medium were analyzed for 23 selected cytokines using the Multiplex Cytokine/Chemokine Panel II Assay (Merck Millipore®). The results were categorized into two groups (top-quality and non-top-quality embryos). The median age of the 39 patients was 34 years. Nine of 23 cytokines were quantified and compared between the top-quality embryo group and non-top-quality embryo group. Among the nine cytokines, CCL15, CCL27, and CXCL-12 were significantly elevated in the top-quality embryo group. These results suggested that specific cytokines measured in human embryo culture media can be used to predict embryo quality and IVF outcomes.
Full Text
https://www.sciencedirect.com/science/article/pii/S1043466621003033
DOI
10.1016/j.cyto.2021.155714
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hye In(김혜인) ORCID logo https://orcid.org/0000-0001-7961-5988
Kim, Heeyon(김희연)
Park, Yun Jeong(박윤정)
Park, Joo Hyun(박주현)
Baek, Hye Won(백혜원)
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
Ahn, So Hyun(안소현)
Yun, Bo Hyon(윤보현) ORCID logo https://orcid.org/0000-0001-5703-797X
Lee, Byung Seok(이병석) ORCID logo https://orcid.org/0000-0001-6001-2079
Lee, Inha(이인하) ORCID logo https://orcid.org/0000-0003-4869-6281
Lee, Jae Hoon(이재훈) ORCID logo https://orcid.org/0000-0003-4223-1395
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
Choi, Young Sik(최영식) ORCID logo https://orcid.org/0000-0002-1157-4822
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186873
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