Interleukin - 6 가 배양된 정맥내피세포에서 E - selectin 의 표현에 미치는 영향

Abstract

Interleukin-6(IL-6) is a pleomorphic cytokine , which has a variety of functions including stimulatory effect of hepatocytes to produce acute phase reactants . With interleukin - l(IL - 1) and tumor necrosis factor - a( TNF ? A ) , IL-6 has a function in acute inflammation. We conducted this study to clarified that IL-6 also has a function to stimulate the expression of E-selectin on cultured HUVEC like IL-1 and TNF-a. The results were as follows ; l. IL-6 had a stimulatory effect to express E - selectin on HUVEC with dose-dependent pattern. 2. A peak stimulatory effect of IL - 6 on expression of E - selectin was 24 hours after incubation and then decreased gradually. 3. This stimulatory effect of IL - 6 on expression of E-selectin was blocked by mouse anti-IL-6 monoclonal antibody. 4. A stimulatory effect of IL-6 on E-selectin expression could be also observed on FACS analysis. 5. When incubated with IL - 6 for 16 hours , the morphology of HUVEC showed shrunken cells, vacuoles in cytoplasms , and some necrosis which looked like a inflammatory response .