위암의 DNA 배수성과 휴지기 세포 분획의 임상 및 병리적 연관성

Abstract

Background/Aims: Previous studies have shown that clinicopathological findings are correlated with the DNA ploidy and S-phase fraction analyzed by flow cytometry in malignant tumors such as breast and colorectal cancer. We studied the clinicopathological correlations of DNA ploidy and S-phase fraction in gastric carcinoma. Methods: DNA flow cytometry was performed using fresh tissue in 188 patients who underwent gastrectomy from Aug. 1993 to Feb. 1995. The DNA ploidy and S-phase fraction were correlated with various clinicopathological findings in a retrospective manner. Results: The incidence of DNA aneuploid was 31.9%. DNA aneuploidy was more frequently associated with male patients and with antral location(p=0.001 & p=0.04). Moderately differentiated adenocarcinoma had a significantly higher frequency of DNA aneuploid than the other histologic type(p=0.007). The overall mean of S-phase fraction was 10.0%, The mean for diploid group was 6.3 fo and that for aneuploid group 17.6%. Higher S-phase fraction(-- 11%) was associated with aneuploid, antral location, moderately differentiated adenocarcinoma, and the group with more than 4 metastatic lymph nodes. Conclusions: DNA aneuploidy and S-phase fraction of gastric carcinoma were significantly correlated with various clinicopathological findings. Further studies on correlations with 1ong-term results such as recurrence and survival rate will be required to establish DNA aneuploidy and S-phase fraction as a significant biological marker in gastric carcinoma