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백서 후각점막 및 호흡점막에서 발육에 다른 씨알릭산의 분포및 Neuraminidase처치후의 변화

Other Titles
 Distribution of Sialoglycoconjugates and Their Polultimate Sugar in Developing Murine Olfactory and Respiratory Mucosa 
Authors
 이정권  ;  윤주헌  ;  김유현  ;  김경수  ;  최진우  ;  김창훈 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.38(7) : 1042-1048, 1994-07 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
ISSN
 1225-035X 
Issue Date
1994-07
Keywords
Sialoglycoconjugates ; Nasal mucosa
Abstract
The functional roles of sialoglycoconjugates are to mediate cell adherence, to control the viscoelasticity of mucus and to serve as receptor sites for the binding of exogenous macromolecules such as those of bacterial or viral etiology. The purpose of this paper is to investigate the distribution of sialoglycoconjugates and their penultimate sugar in developing murine nasal mucosa. Nasal cavities of mice (BALB/c) were fixed by intracardiac perfusion with 2.0% glutaraldehyde and embedded in Epon812.
The serially sectioned slides were stained with Maackia amurensis (MAA) and Sambucus nigra (SNA). And then, the other slides were stained with Dolichos biflorus (DBA) and Arachis hypogaea (PNA) before and after neuraminidase treatment. In olfactory mucosa, all cell surfaces and Bowman's glands were labelled with MAA and SNA from sixteenth gestational day. All cell surfaces were labelled with DBA after neuraminidase treatment. In respiraroty mucosa, all cell surfaces were labelled with SNA from the sixteenth gestational dav. Cilia on a minority of ciliated cells in the mucosa of the septum and nasal floor were stained with MAA. Cell surfaces of respiratory mucosa were labelled with PNA after neuraminidase digestion. However, goblet cells were not labelled with MAA and SNA regardless of aging. Our results indicate that there are different carbohydrate structures of sialoglycoconjugates in the olfactory and respiratory mucosa.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186268
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