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Changes of 5-hydroxytryptamine release by different glucose concentration from hippocampal slices exposed to hypoxia

Authors
 Dong Won Kang  ;  Young Soo Ahn 
Citation
 YONSEI MEDICAL JOURNAL, Vol.36(3) : 271-277, 1995-04 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
1995-04
MeSH
Animals ; Cell Hypoxia ; Dose-Response Relationship, Drug ; Glucose / pharmacology* ; Hippocampus / metabolism* ; In Vitro Techniques ; Male ; Rats ; Rats, Sprague-Dawley ; Serotonin / metabolism*
Keywords
5-hydroxytryptamine ; glucose ; hypoxia ; hippocampal slice
Abstract
We have previously reported that the spontaneous release of [3H]5-hydroxytryptamine(5-HT) was markedly decreased by hypoxic insult in rat hippocampal slices. In the present study, the effect of glucose on 5-HT release was examined. Fractional release of [3H]5-HT was measured from an incubation medium exchanged every 10 min for 140 min and after stabilization of [3H]5-HT release, 10 or 20 min period of hypoxia was induced by exchanging the media which have been previously saturated by 95% N2/5% CO2 gas. In the media containing 1, 2, 5, 10 or 20 mM glucose, [3H]5-HT release was stabilized after 40 min of incubation. Exposure to hypoxia decreased [3H]5-HT release up to 60% of the control level in a glucose concentration-dependent manner and recovered gradually after hypoxic periods. However, in the media containing no glucose, the spontaneous release of [3H] 5-HT increased continuously during incubation. Moreover, when hippocampal slices were exposed to hypoxia, the [3H]5-HT release increased up to 150% of the control level and recovered gradually to the control level after hypoxic periods. These results demonstrate that hypoxia inhibits or enhances 5-HT release in the presence or absence of extracellular glucose respectively and suggest that the availability of extracellular glucose is a key factor to determine the direction of 5-HT release under hypoxic condition.
Files in This Item:
T199500586.pdf Download
DOI
10.3349/ymj.1995.36.3.271
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Ahn, Young Soo(안영수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/186068
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