Cited 0 times in
Changes of 5-hydroxytryptamine release by different glucose concentration from hippocampal slices exposed to hypoxia
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 안영수 | - |
dc.date.accessioned | 2021-12-27T16:41:52Z | - |
dc.date.available | 2021-12-27T16:41:52Z | - |
dc.date.issued | 1995-04 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/186068 | - |
dc.description.abstract | We have previously reported that the spontaneous release of [3H]5-hydroxytryptamine(5-HT) was markedly decreased by hypoxic insult in rat hippocampal slices. In the present study, the effect of glucose on 5-HT release was examined. Fractional release of [3H]5-HT was measured from an incubation medium exchanged every 10 min for 140 min and after stabilization of [3H]5-HT release, 10 or 20 min period of hypoxia was induced by exchanging the media which have been previously saturated by 95% N2/5% CO2 gas. In the media containing 1, 2, 5, 10 or 20 mM glucose, [3H]5-HT release was stabilized after 40 min of incubation. Exposure to hypoxia decreased [3H]5-HT release up to 60% of the control level in a glucose concentration-dependent manner and recovered gradually after hypoxic periods. However, in the media containing no glucose, the spontaneous release of [3H] 5-HT increased continuously during incubation. Moreover, when hippocampal slices were exposed to hypoxia, the [3H]5-HT release increased up to 150% of the control level and recovered gradually to the control level after hypoxic periods. These results demonstrate that hypoxia inhibits or enhances 5-HT release in the presence or absence of extracellular glucose respectively and suggest that the availability of extracellular glucose is a key factor to determine the direction of 5-HT release under hypoxic condition. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Hypoxia | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Glucose / pharmacology* | - |
dc.subject.MESH | Hippocampus / metabolism* | - |
dc.subject.MESH | In Vitro Techniques | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Serotonin / metabolism* | - |
dc.title | Changes of 5-hydroxytryptamine release by different glucose concentration from hippocampal slices exposed to hypoxia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | Dong Won Kang | - |
dc.contributor.googleauthor | Young Soo Ahn | - |
dc.identifier.doi | 10.3349/ymj.1995.36.3.271 | - |
dc.contributor.localId | A02246 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 7660678 | - |
dc.subject.keyword | 5-hydroxytryptamine | - |
dc.subject.keyword | glucose | - |
dc.subject.keyword | hypoxia | - |
dc.subject.keyword | hippocampal slice | - |
dc.contributor.alternativeName | Ahn, Young Soo | - |
dc.contributor.affiliatedAuthor | 안영수 | - |
dc.citation.volume | 36 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 271 | - |
dc.citation.endPage | 277 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.36(3) : 271-277, 1995-04 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.