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Three-Dimensional Distribution of Cochlear Macrophages in the Lateral Wall of Cleared Cochlea

Authors
 Seong Hoon Bae  ;  Sang Hyun Kwak  ;  Jee Eun Yoo  ;  Kyu Min Kim  ;  Young Min Hyun  ;  Jae Young Choi  ;  Jinsei Jung 
Citation
 CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, Vol.14(2) : 179-184, 2021-05 
Journal Title
CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
ISSN
 1976-8710 
Issue Date
2021-05
Keywords
Cochlea ; Macrophages ; Spiral Ligament of Cochlea ; Stria Vascularis
Abstract
Objectives: Resident macrophages are well known to be present in the cochlea, but the exact patterns thereof in spiral ligaments have not been discussed in previous studies. We sought to document the distribution of macrophages in intact cochleae using three-dimensional imaging.

Methods: Cochleae were obtained from C-X3-C motif chemokine receptor 1+/GFP mice, and organ clearing was performed. Three-dimensional images of cleared intact cochleae were reconstructed using two-photon microscopy. The locations of individual macrophages were investigated using 100-μm stacked images to reduce bias. Cochlear inflammation was then induced by lipopolysaccharide (LPS) inoculation into the middle ear through the tympanic membrane. Four days after inoculation, three-dimensional images were obtained.

Results: Macrophages were scarce in areas adjacent to the stria vascularis, particularly the area just beneath it even though many have suspected macrophages to be abundant in this area. This finding remained consistent upon LPS-induced cochlear inflammation, despite a significant increase in the number of macrophages, compared to non-treated cochlea.

Conclusion: Resident macrophages in spiral ligaments are scarce in areas adjacent to the stria vascularis.
Files in This Item:
T202102932.pdf Download
DOI
10.21053/ceo.2020.00395
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Sanghyun(곽상현) ORCID logo https://orcid.org/0000-0002-2827-8456
Bae, Seong Hoon(배성훈) ORCID logo https://orcid.org/0000-0001-9243-9392
Jung, Jinsei(정진세) ORCID logo https://orcid.org/0000-0003-1906-6969
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
Hyun, Young-Min(현영민) ORCID logo https://orcid.org/0000-0002-0567-2039
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/184371
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