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Trichomonas vaginalis-secreted cysteinyl leukotrienes promote migration, degranulation and MCP-1 production in mast cells

Authors
 Young Ah Lee  ;  Young Hee Nam  ;  Arim Min  ;  Myeong Heon Shin 
Citation
 PARASITE IMMUNOLOGY, Vol.42(12) : e12789, 2020-12 
Journal Title
PARASITE IMMUNOLOGY
ISSN
 0141-9838 
Issue Date
2020-12
MeSH
Cell Degranulation ; Cell Line ; Cell Movement ; Chemokine CCL2 / metabolism* ; Cysteine / metabolism* ; Humans ; Immunologic Factors / metabolism* ; Leukotrienes / metabolism* ; Mast Cells / metabolism ; Mast Cells / physiology* ; NADPH Oxidase 2 / metabolism ; Receptors, Leukotriene / metabolism ; Signal Transduction ; Trichomonas vaginalis / metabolism*
Keywords
Trichomonas vaginalis ; NADPH oxidase 2 ; cysteinyl leukotrienes ; mast cells ; monocyte chemoattractant protein-1
Abstract
Trichomonas vaginalis, a flagellated extracellular protozoan parasite that infects the human genitourinary tract, is usually transmitted by sexual contact. Our previous study showed that the leukotriene B4 (LTB4 ), a T vaginalis-secreted lipid mediator, induces interleukin (IL)-8 production and promotes mast cell degranulation and migration via BLT1 in human. In this study, we investigated whether T vaginalis produces another leukotrienes and whether it causes increased MCP-1 production, mast cell migration and degranulation by activating mast cells. We found that cysteinyl leukotrienes (CysLTs) were contained in T vaginalis-derived secretory product (TvSP) by ELISA. The TvSP-stimulated human mast cell line (HMC-1) exhibited significantly increased monocyte chemoattractant protein-1 (MCP-1) secretion compared to the unstimulated cells. Inhibition of NOX2 activation of cells by treatment of NOX inhibitor or NOX2 siRNA reduced TvSP-stimulated MCP-1 production in HMC-1 cells. It was also confirmed that the receptor for CysLTs is expressed in mast cells. The CysLT receptor (CysLTR) antagonist inhibited TvSP-stimulated MCP-1 production of mast cells, as well as ROS production, migration and degranulation of mast cells, and reduced phospho-NF-kB expression. These results suggest that T vaginalis-secreted CysLTs promote migration, degranulation and MCP-1 production in human mast cells through CysLT receptor-mediated NOX2 activation.
Full Text
https://onlinelibrary.wiley.com/doi/10.1111/pim.12789
DOI
10.1111/pim.12789
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Nam, Young Hee(남영희)
Min, A Rim(민아림) ORCID logo https://orcid.org/0000-0003-2938-9630
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183819
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