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Trichomonas vaginalis-secreted cysteinyl leukotrienes promote migration, degranulation and MCP-1 production in mast cells

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dc.contributor.author남영희-
dc.contributor.author민아림-
dc.contributor.author신명헌-
dc.contributor.author이영아-
dc.contributor.author민아림-
dc.contributor.author신명헌-
dc.contributor.author이영아-
dc.date.accessioned2021-09-29T00:24:53Z-
dc.date.available2021-09-29T00:24:53Z-
dc.date.issued2020-12-
dc.identifier.issn0141-9838-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/183819-
dc.description.abstractTrichomonas vaginalis, a flagellated extracellular protozoan parasite that infects the human genitourinary tract, is usually transmitted by sexual contact. Our previous study showed that the leukotriene B4 (LTB4 ), a T vaginalis-secreted lipid mediator, induces interleukin (IL)-8 production and promotes mast cell degranulation and migration via BLT1 in human. In this study, we investigated whether T vaginalis produces another leukotrienes and whether it causes increased MCP-1 production, mast cell migration and degranulation by activating mast cells. We found that cysteinyl leukotrienes (CysLTs) were contained in T vaginalis-derived secretory product (TvSP) by ELISA. The TvSP-stimulated human mast cell line (HMC-1) exhibited significantly increased monocyte chemoattractant protein-1 (MCP-1) secretion compared to the unstimulated cells. Inhibition of NOX2 activation of cells by treatment of NOX inhibitor or NOX2 siRNA reduced TvSP-stimulated MCP-1 production in HMC-1 cells. It was also confirmed that the receptor for CysLTs is expressed in mast cells. The CysLT receptor (CysLTR) antagonist inhibited TvSP-stimulated MCP-1 production of mast cells, as well as ROS production, migration and degranulation of mast cells, and reduced phospho-NF-kB expression. These results suggest that T vaginalis-secreted CysLTs promote migration, degranulation and MCP-1 production in human mast cells through CysLT receptor-mediated NOX2 activation.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherWiley-
dc.relation.isPartOfPARASITE IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHCell Degranulation-
dc.subject.MESHCell Line-
dc.subject.MESHCell Movement-
dc.subject.MESHChemokine CCL2 / metabolism*-
dc.subject.MESHCysteine / metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHImmunologic Factors / metabolism*-
dc.subject.MESHLeukotrienes / metabolism*-
dc.subject.MESHMast Cells / metabolism-
dc.subject.MESHMast Cells / physiology*-
dc.subject.MESHNADPH Oxidase 2 / metabolism-
dc.subject.MESHReceptors, Leukotriene / metabolism-
dc.subject.MESHSignal Transduction-
dc.subject.MESHTrichomonas vaginalis / metabolism*-
dc.titleTrichomonas vaginalis-secreted cysteinyl leukotrienes promote migration, degranulation and MCP-1 production in mast cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorYoung Ah Lee-
dc.contributor.googleauthorYoung Hee Nam-
dc.contributor.googleauthorArim Min-
dc.contributor.googleauthorMyeong Heon Shin-
dc.identifier.doi10.1111/pim.12789-
dc.contributor.localIdA01258-
dc.contributor.localIdA01405-
dc.contributor.localIdA02099-
dc.contributor.localIdA02959-
dc.contributor.localIdA01405-
dc.contributor.localIdA02099-
dc.contributor.localIdA02959-
dc.relation.journalcodeJ02466-
dc.identifier.eissn1365-3024-
dc.identifier.pmid32881004-
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/10.1111/pim.12789-
dc.subject.keywordTrichomonas vaginalis-
dc.subject.keywordNADPH oxidase 2-
dc.subject.keywordcysteinyl leukotrienes-
dc.subject.keywordmast cells-
dc.subject.keywordmonocyte chemoattractant protein-1-
dc.contributor.alternativeNameNam, Young Hee-
dc.contributor.affiliatedAuthor남영희-
dc.contributor.affiliatedAuthor민아림-
dc.contributor.affiliatedAuthor신명헌-
dc.contributor.affiliatedAuthor이영아-
dc.contributor.affiliatedAuthor민아림-
dc.contributor.affiliatedAuthor신명헌-
dc.contributor.affiliatedAuthor이영아-
dc.citation.volume42-
dc.citation.number12-
dc.citation.startPagee12789-
dc.identifier.bibliographicCitationPARASITE IMMUNOLOGY, Vol.42(12) : e12789, 2020-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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