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Trichomonas vaginalis-secreted cysteinyl leukotrienes promote migration, degranulation and MCP-1 production in mast cells
DC Field | Value | Language |
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dc.contributor.author | 남영희 | - |
dc.contributor.author | 민아림 | - |
dc.contributor.author | 신명헌 | - |
dc.contributor.author | 이영아 | - |
dc.date.accessioned | 2021-09-29T00:24:53Z | - |
dc.date.available | 2021-09-29T00:24:53Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 0141-9838 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183819 | - |
dc.description.abstract | Trichomonas vaginalis, a flagellated extracellular protozoan parasite that infects the human genitourinary tract, is usually transmitted by sexual contact. Our previous study showed that the leukotriene B4 (LTB4 ), a T vaginalis-secreted lipid mediator, induces interleukin (IL)-8 production and promotes mast cell degranulation and migration via BLT1 in human. In this study, we investigated whether T vaginalis produces another leukotrienes and whether it causes increased MCP-1 production, mast cell migration and degranulation by activating mast cells. We found that cysteinyl leukotrienes (CysLTs) were contained in T vaginalis-derived secretory product (TvSP) by ELISA. The TvSP-stimulated human mast cell line (HMC-1) exhibited significantly increased monocyte chemoattractant protein-1 (MCP-1) secretion compared to the unstimulated cells. Inhibition of NOX2 activation of cells by treatment of NOX inhibitor or NOX2 siRNA reduced TvSP-stimulated MCP-1 production in HMC-1 cells. It was also confirmed that the receptor for CysLTs is expressed in mast cells. The CysLT receptor (CysLTR) antagonist inhibited TvSP-stimulated MCP-1 production of mast cells, as well as ROS production, migration and degranulation of mast cells, and reduced phospho-NF-kB expression. These results suggest that T vaginalis-secreted CysLTs promote migration, degranulation and MCP-1 production in human mast cells through CysLT receptor-mediated NOX2 activation. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley | - |
dc.relation.isPartOf | PARASITE IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Cell Degranulation | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Movement | - |
dc.subject.MESH | Chemokine CCL2 / metabolism* | - |
dc.subject.MESH | Cysteine / metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunologic Factors / metabolism* | - |
dc.subject.MESH | Leukotrienes / metabolism* | - |
dc.subject.MESH | Mast Cells / metabolism | - |
dc.subject.MESH | Mast Cells / physiology* | - |
dc.subject.MESH | NADPH Oxidase 2 / metabolism | - |
dc.subject.MESH | Receptors, Leukotriene / metabolism | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Trichomonas vaginalis / metabolism* | - |
dc.title | Trichomonas vaginalis-secreted cysteinyl leukotrienes promote migration, degranulation and MCP-1 production in mast cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Young Ah Lee | - |
dc.contributor.googleauthor | Young Hee Nam | - |
dc.contributor.googleauthor | Arim Min | - |
dc.contributor.googleauthor | Myeong Heon Shin | - |
dc.identifier.doi | 10.1111/pim.12789 | - |
dc.contributor.localId | A01258 | - |
dc.contributor.localId | A01405 | - |
dc.contributor.localId | A02099 | - |
dc.contributor.localId | A02959 | - |
dc.relation.journalcode | J02466 | - |
dc.identifier.eissn | 1365-3024 | - |
dc.identifier.pmid | 32881004 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/pim.12789 | - |
dc.subject.keyword | Trichomonas vaginalis | - |
dc.subject.keyword | NADPH oxidase 2 | - |
dc.subject.keyword | cysteinyl leukotrienes | - |
dc.subject.keyword | mast cells | - |
dc.subject.keyword | monocyte chemoattractant protein-1 | - |
dc.contributor.alternativeName | Nam, Young Hee | - |
dc.contributor.affiliatedAuthor | 남영희 | - |
dc.contributor.affiliatedAuthor | 민아림 | - |
dc.contributor.affiliatedAuthor | 신명헌 | - |
dc.contributor.affiliatedAuthor | 이영아 | - |
dc.citation.volume | 42 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | e12789 | - |
dc.identifier.bibliographicCitation | PARASITE IMMUNOLOGY, Vol.42(12) : e12789, 2020-12 | - |
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