황연, 후박 및 구연산 혼합제재물에 의한 Streptococcus mutans의 증식 및 hydroxyapatite 비드 부착 억제효과

Abstract

Streptococcus mutans is an etiologic agent of dental caries and has been known to induce dental caries by the process of initial attachment, proliferation and acid production. For the prevention of dental caries which is caused by this process, extracts of natural products have recently been introduced. To investigate the possibility of using Coptidis Rhizoma and Magnoliae cortex which are natural products as anticariogenic agents, we isolated extracts from Coptidis Rhizoma and Magnoliae Cortex and investigated the inhibitory effect of these extracts on growth of S. mutans using agar diffusion method. And we made several mixtures of Coptidis Rhizoma, Magnoliae Cortex, citric acid and metaphosphate to reduce a bitter taste of Coptidis Rhizoma and Magnoliae Cortex and investigated as follows. After we selected a mixture which had high growth inhibitory activity against S.

mutans by agar diffusion method, we examined the effect of the selected mixture on the attachment of S. mutans to hydroxyapatitie beads and on the growth of S. mutans according to exposure time. A mixture of Coptidis Rhizoma, Magnoliae Cortex and citric acid which combined at 3: 4: 3 ratio presented wide growth inhibitory zone of S. mutans and inhibited the attachment of S. mutans to hydroxyapatite beads at 0.1 % concentration. 0.1 % mixture inhibited growth of S. mutans after 2 hour exposure and 0.5 % mixture inhibited growth of S. mutans after 30 minute exposure. In conclusion, a mixture of Coptidis Rhizoma, Magnoliae Cortex and citric acid turned out to be an effective anticariogenic agent because this mixture inhibited not only growth but also attachment of S. mutans which is the etiologic agent of dental caries.