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Compressive stress induces collective migration through cytoskeletal remodelling in nasal polyp epithelium

Authors
 J M Chung  ;  S G Lee  ;  J-S Nam  ;  J-G Ha  ;  J H Chung  ;  H-J Cho  ;  C-H Kim  ;  S-N Lee  ;  H Lee  ;  J-H Yoon 
Citation
 RHINOLOGY, Vol.59(1) : 49-58, 2021-02 
Journal Title
 RHINOLOGY 
ISSN
 0300-0729 
Issue Date
2021-02
MeSH
Epithelial Cells ; Epithelium ; Humans ; Nasal Cavity ; Nasal Mucosa ; Nasal Polyps*
Abstract
Background: Nasal polyps in the nasal cavity and mucous discharge inside the maxillary sinus exhibit compressive stress on the nasal mucosal epithelium. However, there have been only a few studies on how compressive stress impacts the human nasal mucosal epithelium. Methodology: We investigated the effect of compressive stress on collective migration, junctional proteins, transepithelial electri- cal resistance, epithelial permeability, and gene expression in well-differentiated normal human nasal epithelial (NHNE) cells and human nasal polyp epithelial (HNPE) cells. Results: NHNE cells barely showed collective migration at compressive stress up to 150 mmH20. However, HNPE cells showed much greater degree of collective migration at a lower compressive stress of 100 mmH20. The cell migration of HNPE cells sub- jected to 100 mmH2O compression was significantly decreased at day 3 and was recovered to the status prior to the compressive stress by day 7, indicating that HNPE cells are relatively more sensitive to mechanical pressure than NHNE cells. Compressive stress also increased transepithelial electrical resistance and decreased epithelial permeability, indicating that the compressive stress disturbed the structural organization rather than physical interactions between cells. In addition, we found that compressive stress induced gene expressions relevant to airway inflammation and tissue remodelling in HNPE cells. Conclusion: Taken together, these findings demonstrate that compressive stress on nasal polyp epithelium is capable of inducing collective migration and induce increased expression of genes related to airway inflammation, innate immunity, and polyp remo- delling, even in the absence of inflammatory mediators.
Full Text
https://www.rhinologyjournal.com/Abstract.php?id=2548
DOI
10.4193/Rhin19.268
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Nam, Jae Sung(남재성)
Yoon, Joo Heon(윤주헌)
Lee, Sang Nam(이상남)
Cho, Hyung Ju(조형주) ORCID logo https://orcid.org/0000-0002-2851-3225
Ha, Jong Gyun(하종균) ORCID logo https://orcid.org/0000-0002-2712-1297
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182979
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