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Comparison of Allergenic Properties among Commercially Available House Dust Mite Allergen Extracts in Korea

Authors
 Ji Tae Kim  ;  Hyunho Kim  ;  Sung Hyun Kim  ;  Dong Jun Kim  ;  Yeji Shin  ;  Jung Dong Kim  ;  Hangyeol Song  ;  Seok Woo Jang  ;  Deug Chan Lee  ;  Kyung Hee Park  ;  Jae Hyun Lee  ;  Kyoung Yong Jeong  ;  Jung Won Park 
Citation
 YONSEI MEDICAL JOURNAL, Vol.62(1) : 86-90, 2021-01 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2021-01
MeSH
Allergens / immunology* ; Animals ; Antigens, Dermatophagoides / immunology* ; Desensitization, Immunologic ; Humans ; Hypersensitivity / etiology* ; Hypersensitivity / therapy ; Pyroglyphidae / immunology ; Republic of Korea
Keywords
Allergen ; house dust mite ; immunotherapeutics ; potency ; standardization
Abstract
Precise allergy diagnosis and effective allergen specific immunotherapy are largely dependent on the quality of allergen extract. A new extract of Dermatophagoides farinae was commercially developed by Prolagen. The allergenic properties of the new extract were compared with those of other commercial products. The allergenic properties of the new extract were compared according to protein concentration, protein profiles, major allergen (Der f 1) contents, and allergenic potency to those for three commercially available extracts imported in Korea (Jubilant HollisterStier Allergy, Lofarma S.p.A., and Stallergenes Greer). Protein concentrations varied up to 2.62-fold (0.404 to 1.057 mg/mL), and Der f 1 contents varied up to 11.3-fold (3.597 to 40.688 μg/mL). Protein profiles of the extracts showed no major discrepancies, although there were some differences in SDS-PAGE band intensities, reflecting protein concentrations. Allergen potency ranged from 37038 to 60491 PAU/mL. The Prolagen product was highest in terms of protein concentration and allergen potency. The Lofarma product displayed Der f 1 content similar to that in Prolagen (19.4 μg/mg vs. 19.3 μg/mg). Endotoxin levels varied 8.9-fold (1020 to 8985 EU/mL). The newly developed house dust mite extract showed equal or better allergenic properties than available commercial extracts. This new product may be useful for better diagnostics and allergen-specific immunotherapeutics.
Files in This Item:
T202100160.pdf Download
DOI
10.3349/ymj.2021.62.1.86
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Hee(박경희) ORCID logo https://orcid.org/0000-0003-3605-5364
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181994
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