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Neutrophils infiltrate into the spiral ligament but not the stria vascularis in the cochlea during lipopolysaccharide-induced inflammation

Authors
 Bae, Seong Hoon  ;  Yoo, Jee Eun  ;  Choe, Young Ho  ;  Kwak, Sang Hyun  ;  Choi, Jae Young  ;  Jung, Jinsei  ;  Hyun, Young-Min 
Citation
 THERANOSTICS, Vol.11(6) : 2522-2533, 2021-01 
Journal Title
THERANOSTICS
ISSN
 1838-7640 
Issue Date
2021-01
Keywords
two-photon intravital imaging ; neutrophil ; cochlea ; spiral ligament ; stria vascularis
Abstract
It has been challenging to apply intravital imaging for monitoring the inner ear, as the anatomical location and intricate structure hamper the access of imaging instruments to the inner ear of live mice. By employing intravital imaging of the cochlea in live mice with two-photon microscopy, we investigated neutrophil infiltration into the cochlea tissue and its characteristics under a lipopolysaccharide (LPS)-induced inflammatory state. Methods: Cochlea inflammation was induced by LPS injection to the middle ear. Using two-photon intravital microscopy with specifically designed surgical exteriorization of the cochlea in live mice, we investigated the dynamic features of neutrophils in the lateral wall of the cochlea. The molecular expression pattern of the cochlea lateral wall was also investigated during the LPS-induce inflammation. Results: Despite the contention of whether neutrophils are recruited to the spiral ligament (SL) during inflammation, we observed that LPS-induced inflammation of the middle ear, which mimics acute otitis media, triggered neutrophil migration to the SL in the lateral wall. Notably, massive neutrophil infiltration to the SL occurred 2 days after LPS inoculation, but there was no neutrophil infiltration into the stria vascularis (SV) region. At 1 day after LPS-induced cochlear inflammation, increased mRNA expression of interleukin-1 beta, interleukin-6 were identified in both the SL and SV, while the ICAM-1 mRNA expression increased only in the SL. The differential reactivity of ICAM-1 is likely responsible for the different neutrophil recruitment pattern in the cochlea. Conclusion: Intravital imaging of the cochlea revealed that neutrophil recruitment and infiltration during inflammation are spatially controlled and exclusively observed in the SL but not in the SV and organ of Corti.
Files in This Item:
T202100060.pdf Download
DOI
10.7150/thno.49121
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Sanghyun(곽상현)
Bae, Seong Hoon(배성훈) ORCID logo https://orcid.org/0000-0001-9243-9392
Jung, Jinsei(정진세)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
Hyun, Young-Min(현영민) ORCID logo https://orcid.org/0000-0002-0567-2039
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181872
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