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Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development

Authors
 Yam Prasad Aryal  ;  Eui-Seon Lee  ;  Tae-Young Kim  ;  Shijin Sung  ;  Ji-Youn Kim  ;  Seo-Young An  ;  Jae-Kwang Jung  ;  Jung-Hong Ha  ;  Jo-Young Suh  ;  Hitoshi Yamamoto  ;  Wern-Joo Sohn  ;  Sung-Won Cho  ;  Youngkyun Lee  ;  Chang-Hyeon An  ;  Jae-Young Kim 
Citation
 GENE EXPRESSION PATTERNS, Vol.37 : 119130, 2020-09 
Journal Title
 GENE EXPRESSION PATTERNS 
ISSN
 1567-133X 
Issue Date
2020-09
Keywords
Atf6 ; Ire1 ; Perk ; Tooth morphogenesis ; Xbp1
Abstract
The endoplasmic reticulum (ER) is a site where protein folding and posttranslational modifications occur, but when unfolded or misfolded proteins accumulate in the ER lumen, an unfolded protein response (UPR) occurs. A UPR activates ER-stress signalling genes, including inositol-requiring enzyme-1 (Ire1), activating transcription factor 6 (Atf6), and double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase (Perk), to maintain homeostasis. The involvement of ER stress molecules in metabolic disease and hard tissue matrix formation has been established; however, an understanding of the role of ER-stress signalling molecules in tooth development is lacking. The aims of this study are to define the stage-specific expression patterns of ER stress-related molecules and to elucidate their putative functions in the organogenesis of teeth. This study leverages knowledge of the tissue morphology and expression patterns of a range of signalling molecules during tooth development. RT-qPCR, in situ hybridization, and immunohistochemistry analyses were performed to determine the stage-specific expression patterns of ER-stress-related signalling molecules at important stages of tooth development. RT-qPCR analyses showed that Atf6 and Perk have similar expression levels during all stages of tooth development; however, the expression levels of Ire1 and its downstream target X-box binding protein (Xbp1) increased significantly from the cap to the secretory stage of tooth development. In situ hybridization results revealed that Atf6 and Xbp1 were expressed in cells that form the enamel knot at cap stage and ameloblasts and odontoblasts at secretory stage in stage-specific patterns. In addition, Atf6, Ire1, and Xbp1 expression exhibited distinct localization patterns in secretory odontoblasts and ameloblasts of PN0 molars. Overall, our results strongly suggest that ER-stress molecules are involved in tooth development in response to protein overload that occurs during signaling modulations from enamel knots at cap stage and extracellular matrix secretion at secretory stage.
Full Text
https://www.sciencedirect.com/science/article/pii/S1567133X2030017X
DOI
10.1016/j.gep.2020.119130
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181566
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