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Labeling-free detection of ECD-HER2 protein using aptamer-based nano-plasmonic sensor

Authors
 Jung-Hoon Kim  ;  Jin-Suck Suh  ;  Jaemoon Yang 
Citation
 NANOTECHNOLOGY, Vol.31(17) : 175501, 2020-04 
Journal Title
 NANOTECHNOLOGY 
ISSN
 0957-4484 
Issue Date
2020-04
Keywords
Animals ; Aptamers, Nucleotide / chemistry* ; Biosensing Techniques ; Dopamine / chemistry* ; Gold ; Limit of Detection ; Male ; Metal Nanoparticles ; Rats ; Receptor, ErbB-2 / analysis* ; Receptor, ErbB-2 / blood ; Surface Plasmon Resonance
Abstract
A gold nanoparticle-based localized surface plasmon resonance substrate has been developed as nano-sensors for various bio-applications. However, reproducible and robust sensing substrates anchored gold nanoparticles has not yet been explored. In this study, dopamine-coated gold nanorods (DGNRs) were prepared and immobilized onto the micro-grooving PDMS substrates (mgPDMS). Subsequently, HER2-specific aptamers were conjugated with DGNR/mgPDMS for ECD-HER2 detection. By screening of the optimal concentration of DGNR and aptamers, the effective HER2-specific aptasensor was built up. In particular, the real-time binding assay for the evaluation of limit-of-detection (<5 ng ml-1) was conducted. Furthermore, the binding kinetics for ECD-HER2 was investigated under the biological fluid using a rat serum. Our HER2-specific aptasensor demonstrated the effective sensitivity and selectivity for ECD-HER2.
Full Text
https://iopscience.iop.org/article/10.1088/1361-6528/ab68fa
DOI
10.1088/1361-6528/ab68fa
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180553
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