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Interleukin-4 stimulates lipogenesis in meibocytes by activating the STAT6/PPARγ signaling pathway

Authors
 Ikhyun Jun  ;  Bo-Rahm Kim  ;  Sun Young Park  ;  Hun Lee  ;  Jiyeon Kim  ;  Eung Kweon Kim  ;  Kyoung Yul Seo  ;  Tae-Im Kim 
Citation
 OCULAR SURFACE, Vol.18(4) : 575-582, 2020-10 
Journal Title
 OCULAR SURFACE 
ISSN
 1542-0124 
Issue Date
2020-10
Keywords
Interleukin-4 ; Lipogenesis ; Meibomian gland ; PPARγ ; STAT6
Abstract
Purpose: This study was performed to investigate the effects of interleukin (IL)-4 on adipogenesis and the underlying molecular mechanisms in human meibomian gland epithelial cells (HMGECs). Methods: HMGECs and human white preadipocytes (HWPs) were cultured and differentiated with or without IL-4. Oil-red O staining, Adipored assay, and LipidTox immunostaining were performed to examine the extent of lipid droplet formation. The expression of signal transducer and activator of transcription 6 (STAT6), phospho-STAT6, peroxisome proliferator activator receptor (PPAR)γ, and sterol regulatory element-binding protein (SREBP)-1 was measured through immunoblotting. Cells were treated with STAT6 inhibitor, which prevents the phosphorylation of STAT6 by IL-4, to determine whether the effects of IL-4 on lipogenesis were altered. Results: Treatment with IL-4 significantly facilitated lipid production in differentiated HMGECs. Phosphorylation of STAT6 and expression of key adipogenesis-related molecules PPARγ and SREBP-1 were increased after IL-4 treatment. Inhibition of STAT6 phosphorylation suppressed IL-4-mediated lipid synthesis in HMGECs. In contrast, the lipid synthetic effects of IL-4 were not observed in differentiated HWPs. Conclusions: IL-4 appears to promote lipid synthesis in meibomian gland epithelial cells through the STAT6/PPARγ signaling pathway. This mechanism can serve as a potential therapeutic target for meibomian gland dysfunction.
Full Text
https://www.sciencedirect.com/science/article/pii/S1542012420300781
DOI
10.1016/j.jtos.2020.04.015
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Kim, Tae-Im(김태임) ORCID logo https://orcid.org/0000-0001-6414-3842
Seo, Kyoung Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
Jun, Ik Hyun(전익현) ORCID logo https://orcid.org/0000-0002-2160-1679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179930
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