Cited 20 times in
Interleukin-4 stimulates lipogenesis in meibocytes by activating the STAT6/PPARγ signaling pathway
DC Field | Value | Language |
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dc.contributor.author | 김응권 | - |
dc.contributor.author | 김태임 | - |
dc.contributor.author | 서경률 | - |
dc.contributor.author | 전익현 | - |
dc.date.accessioned | 2020-12-01T16:41:44Z | - |
dc.date.available | 2020-12-01T16:41:44Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 1542-0124 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/179930 | - |
dc.description.abstract | Purpose: This study was performed to investigate the effects of interleukin (IL)-4 on adipogenesis and the underlying molecular mechanisms in human meibomian gland epithelial cells (HMGECs). Methods: HMGECs and human white preadipocytes (HWPs) were cultured and differentiated with or without IL-4. Oil-red O staining, Adipored assay, and LipidTox immunostaining were performed to examine the extent of lipid droplet formation. The expression of signal transducer and activator of transcription 6 (STAT6), phospho-STAT6, peroxisome proliferator activator receptor (PPAR)γ, and sterol regulatory element-binding protein (SREBP)-1 was measured through immunoblotting. Cells were treated with STAT6 inhibitor, which prevents the phosphorylation of STAT6 by IL-4, to determine whether the effects of IL-4 on lipogenesis were altered. Results: Treatment with IL-4 significantly facilitated lipid production in differentiated HMGECs. Phosphorylation of STAT6 and expression of key adipogenesis-related molecules PPARγ and SREBP-1 were increased after IL-4 treatment. Inhibition of STAT6 phosphorylation suppressed IL-4-mediated lipid synthesis in HMGECs. In contrast, the lipid synthetic effects of IL-4 were not observed in differentiated HWPs. Conclusions: IL-4 appears to promote lipid synthesis in meibomian gland epithelial cells through the STAT6/PPARγ signaling pathway. This mechanism can serve as a potential therapeutic target for meibomian gland dysfunction. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | OCULAR SURFACE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Interleukin-4 stimulates lipogenesis in meibocytes by activating the STAT6/PPARγ signaling pathway | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | Ikhyun Jun | - |
dc.contributor.googleauthor | Bo-Rahm Kim | - |
dc.contributor.googleauthor | Sun Young Park | - |
dc.contributor.googleauthor | Hun Lee | - |
dc.contributor.googleauthor | Jiyeon Kim | - |
dc.contributor.googleauthor | Eung Kweon Kim | - |
dc.contributor.googleauthor | Kyoung Yul Seo | - |
dc.contributor.googleauthor | Tae-Im Kim | - |
dc.identifier.doi | 10.1016/j.jtos.2020.04.015 | - |
dc.contributor.localId | A00831 | - |
dc.contributor.localId | A01080 | - |
dc.contributor.localId | A01870 | - |
dc.contributor.localId | A03541 | - |
dc.relation.journalcode | J03095 | - |
dc.identifier.eissn | 1937-5913 | - |
dc.identifier.pmid | 32360783 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1542012420300781 | - |
dc.subject.keyword | Interleukin-4 | - |
dc.subject.keyword | Lipogenesis | - |
dc.subject.keyword | Meibomian gland | - |
dc.subject.keyword | PPARγ | - |
dc.subject.keyword | STAT6 | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.contributor.affiliatedAuthor | 김응권 | - |
dc.contributor.affiliatedAuthor | 김태임 | - |
dc.contributor.affiliatedAuthor | 서경률 | - |
dc.contributor.affiliatedAuthor | 전익현 | - |
dc.citation.volume | 18 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 575 | - |
dc.citation.endPage | 582 | - |
dc.identifier.bibliographicCitation | OCULAR SURFACE, Vol.18(4) : 575-582, 2020-10 | - |
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