12 16

Cited 0 times in

Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes

Authors
 Yu Jin Park  ;  Su-Hee Jeon  ;  Hyun-Kyung Kim  ;  Eun Jung Suh  ;  Seung Jun Choi  ;  Sinyoung Kim  ;  Hyun Ok Kim 
Citation
 JOURNAL OF TRANSLATIONAL MEDICINE, Vol.18(1) : 236, 2020-06 
Journal Title
 JOURNAL OF TRANSLATIONAL MEDICINE 
Issue Date
2020-06
Keywords
Induced pluripotent stem cell ; Rare blood type ; Red blood cell differentiation ; Stem cell banking
Abstract
Background: The in vitro production of mature human red blood cells (RBCs) from induced pluripotent stem cells (iPSCs) has been the focus of research to meet the high demand for blood transfusions. However, limitations like high costs and technological requirements restrict the use of RBCs produced by iPSC differentiation to specific circumstances, such as for patients with rare blood types or alloimmunized patients. In this study, we developed a detailed protocol for the generation of iPSC lines derived from peripheral blood of donors with O D-positive blood and rare blood types (D-and Jr(a-)) and subsequent erythroid differentiation. Methods: Mononuclear cells separated from the peripheral blood of O D-positive and rare blood type donors were cultured to produce and expand erythroid progenitors and reprogrammed into iPSCs. A 31-day serum-free, xeno-free erythroid differentiation protocol was used to generate reticulocytes. The stability of iPSC lines was confirmed with chromosomal analysis and RT-PCR. Morphology and cell counts were determined by microscopy observations and flow cytometry. Results: Cells from all donors were successfully used to generate iPSC lines, which were differentiated into erythroid precursors without any apparent chromosomal mutations. This differentiation protocol resulted in moderate erythrocyte yield per iPSC. Conclusions: It has previously only been hypothesized that erythroid differentiation from iPSCs could be used to produce RBCs for transfusion to patients with rare blood types or who have been alloimmunized. Our results demonstrate the feasibility of producing autologous iPSC-differentiated RBCs for clinical transfusions in patients without alternative options.
Files in This Item:
T202002560.pdf Download
DOI
10.1186/s12967-020-02403-y
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Choi, Seung Jun(최승준) ORCID logo https://orcid.org/0000-0003-0736-1055
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179323
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links