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Involvement of Two NF-kB Binding Sites in PMA-induced Expression of the Human Leukotactin-1/CCL15 Gene in U937 Monocytoid Cells

Authors
 Yong-Hyun Shin  ;  Jung-Jae Shim  ;  Man-Wook Hur  ;  Chang-Joong Kang  ;  Jiyoung Kim 
Citation
 MOLECULES AND CELLS, Vol.17(2) : 316-321, 2004-04 
Journal Title
 MOLECULES AND CELLS 
ISSN
 1016-8478 
Issue Date
2004-04
Keywords
CCL15 ; Chemokine ; Gene Expression ; Leukotactin-1 ; Monocytoid Cell ; NF-kB
Abstract
Leukotactin-1 (Lkn-1)/CCL15, is a recently cloned chemotactic chemokine that appears to play important roles in the inflammatory process by recruiting immune cells to inflammatory sites. Expression of the Lkn-1/CCL15 gene is inducible in monocytes but its transcriptional regulation has not been studied. To identify Lkn-1/CCL15 regulatory sequences in monocytic cells, U937 cells were transiently transfected with the luciferase reporter gene linked to various deletions of the Lkn-1/CCL15 promoter region. The region ?269 to ?43 bp from the transcription start site proved to be important for induction by PMA. This region contained two potential NF-kB sites: one between ?191 and ?182 bp, and the other between ?60 and ?51 bp. Mutation of either element reduced PMA-induced expression and electrophoretic mobility shift assays revealed that NF-kB recognized both potential NF-kB sites. In addition, PMA-induction of Lkn-1/CCL15 in transiently transfected U937 cells was blocked by proteasome inhibitor 1. These observations demonstrate that the two NF-kB binding sites are essential for PMA-induced Lkn-1/CCL15 expression in human monocytes.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178785
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