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Involvement of Two NF-kB Binding Sites in PMA-induced Expression of the Human Leukotactin-1/CCL15 Gene in U937 Monocytoid Cells
DC Field | Value | Language |
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dc.contributor.author | 허만욱 | - |
dc.date.accessioned | 2020-09-04T02:04:36Z | - |
dc.date.available | 2020-09-04T02:04:36Z | - |
dc.date.issued | 2004-04 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178785 | - |
dc.description.abstract | Leukotactin-1 (Lkn-1)/CCL15, is a recently cloned chemotactic chemokine that appears to play important roles in the inflammatory process by recruiting immune cells to inflammatory sites. Expression of the Lkn-1/CCL15 gene is inducible in monocytes but its transcriptional regulation has not been studied. To identify Lkn-1/CCL15 regulatory sequences in monocytic cells, U937 cells were transiently transfected with the luciferase reporter gene linked to various deletions of the Lkn-1/CCL15 promoter region. The region ?269 to ?43 bp from the transcription start site proved to be important for induction by PMA. This region contained two potential NF-kB sites: one between ?191 and ?182 bp, and the other between ?60 and ?51 bp. Mutation of either element reduced PMA-induced expression and electrophoretic mobility shift assays revealed that NF-kB recognized both potential NF-kB sites. In addition, PMA-induction of Lkn-1/CCL15 in transiently transfected U937 cells was blocked by proteasome inhibitor 1. These observations demonstrate that the two NF-kB binding sites are essential for PMA-induced Lkn-1/CCL15 expression in human monocytes. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Korean Society for Molecular and Cellular Biology | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Involvement of Two NF-kB Binding Sites in PMA-induced Expression of the Human Leukotactin-1/CCL15 Gene in U937 Monocytoid Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) | - |
dc.contributor.googleauthor | Yong-Hyun Shin | - |
dc.contributor.googleauthor | Jung-Jae Shim | - |
dc.contributor.googleauthor | Man-Wook Hur | - |
dc.contributor.googleauthor | Chang-Joong Kang | - |
dc.contributor.googleauthor | Jiyoung Kim | - |
dc.contributor.localId | A04350 | - |
dc.relation.journalcode | J02273 | - |
dc.identifier.eissn | 0219-1032 | - |
dc.subject.keyword | CCL15 | - |
dc.subject.keyword | Chemokine | - |
dc.subject.keyword | Gene Expression | - |
dc.subject.keyword | Leukotactin-1 | - |
dc.subject.keyword | Monocytoid Cell | - |
dc.subject.keyword | NF-kB | - |
dc.contributor.alternativeName | Hur, Man Wook | - |
dc.contributor.affiliatedAuthor | 허만욱 | - |
dc.citation.volume | 17 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 316 | - |
dc.citation.endPage | 321 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, Vol.17(2) : 316-321, 2004-04 | - |
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