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Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y-chromosomal Short Tandem Repeat Analysis for Archeological Human Bones

Authors
 Chang Seok Oh  ;  Soong Deok Lee  ;  Kyoung-Jin Shin  ;  Dong Hoon Shin 
Citation
 JOURNAL OF FORENSIC SCIENCES, Vol.61(2) : 430-438, 2016-03 
Journal Title
 JOURNAL OF FORENSIC SCIENCES 
ISSN
 0022-1198 
Issue Date
2016-03
Keywords
AmpFℓSTR Yfiler PCR amplification kit ; Joseon ; Korea ; Y-chromosomal short tandem repeat ; Y-miniplex primer ; ancient DNA ; forensic science
Abstract
The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom-designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y-STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom-designed primers). In this study, the authors established that the custom-designed primers offer a markedly improved success rate for obtainment of Y-STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1111/1556-4029.12950
DOI
10.1111/1556-4029.12950
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178484
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