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Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y-chromosomal Short Tandem Repeat Analysis for Archeological Human Bones
DC Field | Value | Language |
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dc.contributor.author | 신경진 | - |
dc.date.accessioned | 2020-07-27T16:43:23Z | - |
dc.date.available | 2020-07-27T16:43:23Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 0022-1198 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178484 | - |
dc.description.abstract | The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom-designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y-STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom-designed primers). In this study, the authors established that the custom-designed primers offer a markedly improved success rate for obtainment of Y-STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Blackwell Pub. | - |
dc.relation.isPartOf | JOURNAL OF FORENSIC SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y-chromosomal Short Tandem Repeat Analysis for Archeological Human Bones | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Forensic Medicine (법의학과) | - |
dc.contributor.googleauthor | Chang Seok Oh | - |
dc.contributor.googleauthor | Soong Deok Lee | - |
dc.contributor.googleauthor | Kyoung-Jin Shin | - |
dc.contributor.googleauthor | Dong Hoon Shin | - |
dc.identifier.doi | 10.1111/1556-4029.12950 | - |
dc.contributor.localId | A02085 | - |
dc.relation.journalcode | J01413 | - |
dc.identifier.eissn | 1556-4029 | - |
dc.identifier.pmid | 26375610 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1111/1556-4029.12950 | - |
dc.subject.keyword | AmpFℓSTR Yfiler PCR amplification kit | - |
dc.subject.keyword | Joseon | - |
dc.subject.keyword | Korea | - |
dc.subject.keyword | Y-chromosomal short tandem repeat | - |
dc.subject.keyword | Y-miniplex primer | - |
dc.subject.keyword | ancient DNA | - |
dc.subject.keyword | forensic science | - |
dc.contributor.alternativeName | Shin, Kyoung Jin | - |
dc.contributor.affiliatedAuthor | 신경진 | - |
dc.citation.volume | 61 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 430 | - |
dc.citation.endPage | 438 | - |
dc.identifier.bibliographicCitation | JOURNAL OF FORENSIC SCIENCES, Vol.61(2) : 430-438, 2016-03 | - |
dc.identifier.rimsid | 64828 | - |
dc.type.rims | ART | - |
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