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저산소/재산소화와 Sodium Nitroprusside가 분리 위선세포의 산분비 및 세포내 Cyclic Nucleotide에 미치는 영향

Other Titles
 Effects of Hypoxia/Reoxygenation and Sodium Nitroprusside on Acid Secretion and Cyclic Nucleotide of Isolated Gastric Cells 
 김혜영  ;  이송철  ;  김경환 
 Korean Journal of Gastroenterology (대한소화기학회지), Vol.29(2) : 164-174, 1997 
Journal Title
 Korean Journal of Gastroenterology (대한소화기학회지) 
Issue Date
Background/Aims: Ischemia/reperfusion is associated with mucosal injury in trauma, rnajor surgery, and nonsteroidal anti-inflammatory drug administration. Nitric oxide and cyclic nucleotides appear to play a cytoprotective role against gastric mucosal injury. Present study was aimed to investigate a cytoprotective mechanism of gastric cells against hypoxia/reoxygenation. Methods: lsolated gastric cells treated with or without sodium nitroprusside were exposed to hypoxia(30min) /reoxygenation(lh). Histamine(]0'M) with 3-isobuty]-1-methylxanthine(10'M) was used to stimulate acid secretion. Acid secretory activity was measured by [ C]-aminopyrine accumulation ratio. The intracellular cGMP and cAMP were deterrnined by radioimmunoassay. The cell viability was assessed by lactic dehydrogenase release and trypan blue exclusion test. Results: Hypoxia/ reoxygenation inhibited acid secretion, but increased intracellular levels of cGMP and cAMP. Sodium nitroprusside inhibited acid secretion stimulated by histamine and 3-isobutyl- 1-methylxanthine. cGMP level, but not cAMP level, of gastric cells was increased by sodium nitroprusside. Cell viability was neither affected by hypoxia/reoxygenation nor sodium nitroprusside. Conclusions: Hypoxia/reoxygenation increases cGMP and inhibits acid secretion, possibly through nitric oxide induction, which acts as a mucosal defense mechanism. Increase of cellular cAMP during hypoxic exposure is thought of as cellular adaptation, and this is not re]ated to acid secretion. (Korean J Gastroenterol 1997;29:164-174)
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1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
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